NF-|[kappa]|B dictates the degradation pathway of I|[kappa]|B|[alpha]|

doi:doi:10.1038/emboj.2008.91

Corrigendum

The EMBO Journal (2008) 27, 1421
doi:10.1038/emboj.2008.91

NF-B dictates the degradation pathway of IB

Erika Mathes, Ellen L O'Dea, Alexander Hoffmann and Gourisankar Ghosh

Correction to:The EMBO Journal, advance online publication 10 April 2008; doi:10.1038/emboj.2008.73

Since the publication of this paper, the authors have noticed an error in Figure 2B. The X-axis should have been labelled in hours and not minutes.

Figure 1.

NF- kappa B determines the degradation pathway of I kappa B alpha . (A) NF- kappa B protects I kappa B alpha from proteasomal degradation in vitro. Top: purified 20S proteasome and I kappa B alpha were incubated at 37°C, with or without purified p65. (I) represents the proteasome inhibitor MG132. (B) I kappa B alpha is highly stable in vivo in the presence of NF- kappa B. Left panel: WB showing WT, IKK phosphorylation and ubiquitination-defective mutants introduced into ikba^-/-, where all NF- kappa B subunits are present. Cells were treated with cycloheximide (CHX) for different lengths of time (up to 24 h) and the protein levels were visualized by WB. Right panel: this experiment was repeated twice and is represented graphically with error bars signifying plusminus s.e.m. ( circle ) Transgenic WT I kappa B alpha , ( square ) K21, 22R I kappa B alpha , ( triangle ) KR9 I kappa B alpha and ( diamond ) S32, 36A I kappa B alpha . (C) A model of NF- kappa B repression by I kappa B alpha in pre-stimulated cells. There are two processes that control I kappa B alpha degradation. In the resting cell, basal IKK activity phosphorylates bound I kappa B alpha and targets it for ubiquitin-dependent degradation. In addition, free I kappa B alpha is continuously synthesized and degraded in an IKK- and Ub-independent mechanism. This keeps NF- kappa B from being activated in the resting cell.