Article
- The EMBO Journal (2008) 27, 1039 - 1048
- doi:10.1038/emboj.2008.43
Published online: 13 March 2008
Subject Category:
The BTG2 protein is a general activator of mRNA deadenylation
Fabienne Mauxion1,2,3, Céline Faux1,2,3 and Bertrand Séraphin1,2,3
- CNRS, Equipe Labellisée La Ligue, Centre de Génétique Moléculaire, UPR 2167, Gif-sur-Yvette, France
- Université Paris-Sud, Orsay, France
- Université Pierre et Marie Curie-Paris 6, Paris, France
Correspondence to:
Bertrand Séraphin, ARN, CGM—CNRS, Avenue de la Terrasse, Gif sur Yvette Cedex 91198, France. Tel.: +33 1 69 82 38 83; Fax: +33 1 69 82 38 77; E-mail: seraphin@cgm.cnrs-gif.fr
Fabienne Mauxion, ARN, CGM—CNRS, Avenue de la Terrasse, Gif sur Yvette Cedex 91198, France. Tel.: +33 1 69 82 38 83; Fax: +33 1 69 82 38 77; E-mail: mauxion@cgm.cnrs-gif.fr
Received 14 August 2007; Accepted 18 February 2008
Abstract
BTG2 is a prototype member of the BTG/Tob family of antiproliferative proteins, originally identified as a primary response gene induced by growth factors and tumour promoters. Its expression has been linked to diverse cellular processes such as cell-cycle progression, differentiation or apoptosis. BTG2 has also been shown to interact with the Pop2/Caf1 deadenylase. Here, we demonstrate that BTG2 is a general activator of mRNA decay, thereby contributing to gene expression control. Detailed characterizations of BTG2 show that it enhances deadenylation of all transcripts tested. Our results demonstrate that Caf1 nuclease activity is required for efficient deadenylation in mammalian cells and that the deadenylase activities of both Caf1 and its Ccr4 partner are required for Btg2-induced poly(A) degradation. General activation of deadenylation may represent a new mode of global regulation of gene expression, which could be important to allow rapid resetting of protein production during development or after specific stresses. This may constitute a common function for BTG/Tob family members.
Keywords:
- BTG,
- deadenylase,
- mRNA decay,
- poly(A),
- Tob
MORE ARTICLES LIKE THIS
These links to content published by NPG are automatically generated
REVIEWS
Multifunctional deadenylase complexes diversify mRNA control
Nature Reviews Molecular Cell Biology Perspective (01 Apr 2008)
NEWS AND VIEWS
Applying the brakes on gene expression
Nature Structural & Molecular Biology News and Views (01 Dec 2005)
RESEARCH
Concerted action of poly(A) nucleases and decapping enzyme in mammalian mRNA turnover
Nature Structural & Molecular Biology Article (01 Dec 2005)
Regulation of ARE transcript 3′ end processing by the yeast Cth2 mRNA decay factor
The EMBO Journal Article (19 Nov 2008)
The EMBO Journal Article (15 Mar 2002)
