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| Subject Categories: Chromatin & Transcription |
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| The EMBO Journal
(2008) 27, 817–826, doi:10.1038/sj.emboj.7601902 Published online 25 October 2007 |
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| ACF catalyses chromatosome movements in chromatin fibres |
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| Verena K Maier1, Mariacristina Chioda1, Daniela Rhodes2 and Peter B Becker1 |
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1 Molekularbiologie, Adolf-Butenandt Institut, Ludwig Maximilian Universität, Munich, Germany 2 Structural Studies, MRC Laboratory of Molecular Biology, Cambridge, UK To whom correspondence should be addressed Peter B Becker, Molekularbiologie, Adolf-Butenandt Institut, Ludwig Maximilians Universität, Schillerstrasse 44, München 80336, Germany. Tel.: +49 89 2180 75427; Fax: +49 89 2180 75425; E-mail: pbecker@med.uni-muenchen.de Received 1 June 2007; Accepted 4 October 2007; Published online 25 October 2007. |
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| Abstract |
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| Nucleosome-remodelling factors containing the ATPase ISWI, such as ACF, render DNA in chromatin accessible by promoting the sliding of histone octamers. Although the ATP-dependent repositioning of mononucleosomes is readily observable in vitro, it is unclear to which extent nucleosomes can be moved in physiological chromatin, where neighbouring nucleosomes, linker histones and the folding of the nucleosomal array restrict mobility. We assembled arrays consisting of 12 nucleosomes or 12 chromatosomes (nucleosomes plus linker histone) from defined components and subjected them to remodelling by ACF or the ATPase CHD1. Both factors increased the access to DNA in nucleosome arrays. ACF, but not CHD1, catalysed profound movements of nucleosomes throughout the array, suggesting different remodelling mechanisms. Linker histones inhibited remodelling by CHD1. Surprisingly, ACF catalysed significant repositioning of entire chromatosomes in chromatin containing saturating levels of linker histone H1. H1 inhibited the ATP-dependent generation of DNA accessibility by only about 50%. This first demonstration of catalysed chromatosome movements suggests that the bulk of interphase euchromatin may be rendered dynamic by dedicated nucleosome-remodelling factors. |
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| Keywords: chromatin, Drosophila, linker histone, nucleosome array, remodelling |
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Top of page MORE ARTICLES LIKE THISThese links to content published by NPG are automatically generated NEWS AND VIEWSNucleosome remodelers on track Nature Structural & Molecular Biology News and Views (01 Sep 2005) Lighting up nucleosome spacing Nature Structural & Molecular Biology News and Views (01 Dec 2006) RESEARCHACF catalyses chromatosome movements in chromatin fibres The EMBO Journal Article SWI/SNF remodeling and p300-dependent transcription of histone variant H2ABbd nucleosomal arrays The EMBO Journal Article (29 Sep 2004) |
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