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| Subject Categories: Chromatin & Transcription |
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| The EMBO Journal
(2008) 27, 394–405, doi:10.1038/sj.emboj.7601966 Published online 10 January 2008 |
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| SAGA-mediated H2B deubiquitination controls the development of neuronal connectivity in the Drosophila visual system |
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| Vikki M Weake, Kenneth K Lee, Sebastián Guelman1, Chia-Hui Lin, Christopher Seidel, Susan M Abmayr and Jerry L Workman |
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Stowers Institute for Medical Research, Kansas City, MO, USA To whom correspondence should be addressed Jerry L Workman, Stowers Institute for Medical Research, 1000 E 50th Street, Kansas City, MO 64110, USA. Tel.: +1 816 926 4392; Fax: +1 816 926 4692; E-mail: jlw@stowers-institute.org 1 Present address: Genentech Inc., 1 DNA Way, South San Francisco, CA 94080-4990, USA Received 14 June 2007; Accepted 30 November 2007; Published online 10 January 2008. |
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| Abstract |
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| Nonstop, which has previously been shown to have homology to ubiquitin proteases, is required for proper termination of axons R1–R6 in the optic lobe of the developing Drosophila eye. Herein, we establish that Nonstop actually functions as an ubiquitin protease to control the levels of ubiquitinated histone H2B in flies. We further establish that Nonstop is the functional homolog of yeast Ubp8, and can substitute for Ubp8 function in yeast cells. In yeast, Ubp8 activity requires Sgf11. We show that in Drosophila, loss of Sgf11 function causes similar photoreceptor axon-targeting defects as loss of Nonstop. Ubp8 and Sgf11 are components of the yeast SAGA complex, suggesting that Nonstop function might be mediated through the Drosophila SAGA complex. Indeed, we find that Nonstop does associate with SAGA components in flies, and mutants in other SAGA subunits display nonstop phenotypes, indicating that SAGA complex is required for accurate axon guidance in the optic lobe. Candidate genes regulated by SAGA that may be required for correct axon targeting were identified by microarray analysis of gene expression in SAGA mutants. |
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| Keywords: H2B deubiquitination, histone acetyltransferase, neural development, Nonstop, SAGA |
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Top of page MORE ARTICLES LIKE THISThese links to content published by NPG are automatically generated RESEARCHThe EMBO Journal Article Dissecting muscle and neuronal disorders in a Drosophila model of muscular dystrophy The EMBO Journal Article Dissecting muscle and neuronal disorders in a Drosophila model of muscular dystrophy The EMBO Journal Article (24 Jan 2007) Control of photoreceptor axon target choice by transcriptional repression of Runt Nature Neuroscience Article (01 Aug 2002) |
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