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Figure 1

Dissecting bold beta-ring assembly pathway of the mammalian 20S proteasome

Yuko Hirano, Takeumi Kaneko, Kenta Okamoto, Minghui Bai, Hideki Yashiroda, Kaori Furuyama, Koichi Kato, Keiji Tanaka and Shigeo Murata

  • The EMBO Journal (2008) 27, 2204 - 2213
  • doi:10.1038/emboj.2008.148

Published online: 24 July 2008

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Accumulation of distinct assembly intermediates in each beta-subunit knockdown cells. The cell extracts (40 mug) used in Supplementary Figure S1 were separated by native PAGE. Assembly intermediates were detected by immunoblotting using the indicated antibodies (A, C–I) The bands corresponding to alpha-ring and the 20S proteasome as well as the locations of molecular size markers are depicted by arrowheads. (B) The peptide-hydrolysing activity was assayed by active staining of the gel using Suc-LLVY-MCA in the presence of SDS. Note that the 26S proteasome did not move inside the native PAGE gel.

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