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The EMBO Journal (2008) 27, 2214–2221, doi:10.1038/emboj.2008.147 Published online 24 July 2008 Figure 2 Arabidopsis MAP kinase 4 regulates gene expression through transcription factor release in the nucleus Jin-Long Qiu, Berthe Katrine Fiil, Klaus Petersen, Henrik Bjørn Nielsen, Christopher J Botanga, Stephan Thorgrimsen, Kristoffer Palma, Maria Cristina Suarez-Rodriguez, Signe Sandbech-Clausen, Jacek Lichota, Peter Brodersen, Klaus D Grasser, Ole Mattsson, Jane Glazebrook, John Mundy and Morten Petersen

Figure 2 WRKY33 binding to and activation from the PAD3 promoter. (A, B) Enrichment of P1 amplicons from chromatin immunoprecipitates with anti-WRKY33 antibodies from regions of the PAD3 promoter over background (measured with primers to a promoter region of the ROC5 control gene) in Col-0 and wrky33 treated with flg22 for 1.5 h (A) and after treatment with Pst DC3000 (avrRpm1) for 4 h (B). The samples were tested in triplicate and normalized to ROC5. Meanss.d. are shown. (C) Transient expression assays using a 2.4-kb fragment of the PAD3 promoter fused to the GUS reporter gene (PAD3:GUS) inoculated with Pst DC3000 (avrRpm1). Bombardments of detached leaves of Col-0 and wrky33 mutant were done with PAD3:GUS in combination with an empty vector or an effector of the CaMV 35S promoter driving WRKY33 or WRKY22 full-length cDNA. A 35S:LUC construct was used as a control for the efficiency of transient transformation. GUS activity in each sample was expressed relative to LUC activity to normalize data for variation in transformation efficiency.

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