Figure 1

Molecular and phenotypic analysis of ETG1-deficient plants. (A) Exon (boxes) and intron (lines) structure of ETG1. Coding and non-coding regions are shown as black and white boxes, respectively. White triangles and arrows indicate T-DNA insertion sites and primer positions used for real-time RT–PCR analysis, respectively. (B) Real-time RT–PCR analysis of ETG1 expression in wild-type (WT), etg1-1, and etg1-2 plants. Total RNA prepared from first leaves of 9-day-old plants was amplified by RT–PCR. All values were normalized against the expression level of the ACTIN2 gene. (C) Seedling phenotypes of 21-day-old wild-type (WT) and etg1-1 plants. (D) Ploidy level distribution of the first leaves of 3-week-old wild-type (WT), etg1-1, and etg1-2 plants as measured by flow cytometry. Data represent averages.d. (n=5). ^*Significant statistical differences by t-test (P<0.05) between wild type and etg1. (E) Drawing-tube image of the first leaves of 3-week-old wild-type (left) and etg1-1 (right) plants. Bar=100 m. (F–H) Leaf growth of the first leaf pair of wild-type (WT), etg1-1, and etg1-2 plants. Leaf blade area (F), epidermal cell number (G), and epidermal cell size (H) on the abaxial side of the leaf. Data represent averages.d. (n=5). (I, J) Adult phenotype of wild-type (WT) and etg1-1 plants. The plants were photographed 5 weeks after germination. (J) Magnification of leaves in wild-type (WT) and etg1-1 plants.