Article
- The EMBO Journal (2008) 27, 1791 - 1803
- doi:10.1038/emboj.2008.112
Published online: 29 May 2008
Subject Categories:
Sequential Cyk-4 binding to ECT2 and FIP3 regulates cleavage furrow ingression and abscission during cytokinesis
Glenn C Simon1, Eric Schonteich1, Christine C Wu2, Alisa Piekny3, Damian Ekiert3,a, Xinzi Yu4, Gwyn W Gould4, Michael Glotzer3 and Rytis Prekeris1
- Department of Cellular and Developmental Biology, School of Medicine, University of Colorado Health Sciences Center, Aurora, CO, USA
- Department of Pharmacology, School of Medicine, University of Colorado Health Sciences Center, Aurora, CO, USA
- Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL, USA
- Henry Wellcome Laboratory of Cell Biology, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK
Correspondence to:
Rytis Prekeris, Department of Cellular and Developmental Biology, School of Medicine, University of Colorado Health Sciences Center, 12801 E 17th Avenue, Aurora, CO 80045, USA. Tel.: +1 303 724 3411; Fax: +1 303 724 3420; E-mail: Rytis.Prekeris@uchsc.edu
aPresent Address: The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
Received 11 December 2007; Accepted 14 May 2008
Abstract
Cytokinesis is a highly regulated and dynamic event that involves the reorganization of the cytoskeleton and membrane compartments. Recently, FIP3 has been implicated in targeting of recycling endosomes to the mid-body of dividing cells and is found required for abscission. Here, we demonstrate that the centralspindlin component Cyk-4 is a FIP3-binding protein. Furthermore, we show that FIP3 binds to Cyk-4 at late telophase and that centralspindlin may be required for FIP3 recruitment to the mid-body. We have mapped the FIP3-binding region on Cyk-4 and show that it overlaps with the ECT2-binding domain. Finally, we demonstrate that FIP3 and ECT2 form mutually exclusive complexes with Cyk-4 and that dissociation of ECT2 from the mid-body at late telophase may be required for the recruitment of FIP3 and recycling endosomes to the cleavage furrow. Thus, we propose that centralspindlin complex not only regulates acto-myosin ring contraction but also endocytic vesicle transport to the cleavage furrow and it does so through sequential interactions with ECT2 and FIP3.
Keywords:
- Cyk-4,
- cytokinesis,
- endosomes,
- FIP3,
- Rab11
MORE ARTICLES LIKE THIS
These links to content published by NPG are automatically generated
REVIEWS
The 3Ms of central spindle assembly: microtubules, motors and MAPs
Nature Reviews Molecular Cell Biology Review (01 Jan 2009)
The 3Ms of central spindle assembly: microtubules, motors and MAPs
Nature Reviews Molecular Cell Biology Review (01 Jan 2009)
NEWS AND VIEWS
Nature Cell Biology News and Views (01 Feb 2009)
Nature Structural & Molecular Biology News and Views (01 Jul 2007)
