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  • The EMBO Journal (2008) 27, 1791 - 1803
  • doi:10.1038/emboj.2008.112

Published online: 29 May 2008

Sequential Cyk-4 binding to ECT2 and FIP3 regulates cleavage furrow ingression and abscission during cytokinesis

Glenn C Simon1, Eric Schonteich1, Christine C Wu2, Alisa Piekny3, Damian Ekiert3,a, Xinzi Yu4, Gwyn W Gould4, Michael Glotzer3 and Rytis Prekeris1

  1. Department of Cellular and Developmental Biology, School of Medicine, University of Colorado Health Sciences Center, Aurora, CO, USA
  2. Department of Pharmacology, School of Medicine, University of Colorado Health Sciences Center, Aurora, CO, USA
  3. Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL, USA
  4. Henry Wellcome Laboratory of Cell Biology, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK

Correspondence to:

Rytis Prekeris, Department of Cellular and Developmental Biology, School of Medicine, University of Colorado Health Sciences Center, 12801 E 17th Avenue, Aurora, CO 80045, USA. Tel.: +1 303 724 3411; Fax: +1 303 724 3420; E-mail: Rytis.Prekeris@uchsc.edu

aPresent Address: The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA

Received 11 December 2007; Accepted 14 May 2008

Cytokinesis is a highly regulated and dynamic event that involves the reorganization of the cytoskeleton and membrane compartments. Recently, FIP3 has been implicated in targeting of recycling endosomes to the mid-body of dividing cells and is found required for abscission. Here, we demonstrate that the centralspindlin component Cyk-4 is a FIP3-binding protein. Furthermore, we show that FIP3 binds to Cyk-4 at late telophase and that centralspindlin may be required for FIP3 recruitment to the mid-body. We have mapped the FIP3-binding region on Cyk-4 and show that it overlaps with the ECT2-binding domain. Finally, we demonstrate that FIP3 and ECT2 form mutually exclusive complexes with Cyk-4 and that dissociation of ECT2 from the mid-body at late telophase may be required for the recruitment of FIP3 and recycling endosomes to the cleavage furrow. Thus, we propose that centralspindlin complex not only regulates acto-myosin ring contraction but also endocytic vesicle transport to the cleavage furrow and it does so through sequential interactions with ECT2 and FIP3.

  • Keywords:

    • Cyk-4,
    • cytokinesis,
    • endosomes,
    • FIP3,
    • Rab11