Figure 1

Organization and Alu characterization of Nicn1 and Lin28. The genomic sequences of Nicn1 (A) and Lin28 (B) are drawn to scale. Exons and UTRs are shown as black bars, with coding regions being thicker. The Alu elements present in these two genes are shown as gray arrows with the indicated orientations. There is a single pair of IRAlus in each of the 3'-UTRs of Nicn1 and Lin28. The small black arrows indicate the PCR primers, their directions and their relative positions for the cloning sequences on the pEGFP-C1 vector. AK094248 shows one of the highly edited mRNA sequences of Nicn1 in the UCSC genome browser, whereas CN349329 shows one of the highly edited mRNA sequences of Lin28. The edited residues are denoted in lowercase in bold.