Figure 3
The Moraxella adhesin UspA1 binds to its human CEACAM1 receptor by a deformable trimeric coiled-coil
Rebecca Conners, Darryl J Hill, Elena Borodina, Christopher Agnew, Sarah J Daniell, Nicholas M Burton, Richard B Sessions, Anthony R Clarke, Lucy E Catto, Donna Lammie, Timothy Wess, R Leo Brady and Mumtaz Virji
- The EMBO Journal (2008) 27, 1779 - 1789
- doi:10.1038/emboj.2008.101
Published online: 22 May 2008
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Circular dichroism (CD) spectra. (A) CD spectra from UspA1(527–665) across a range of pH values. (B) CD spectra of native UspA1(527–665) (solid diamonds) compared with the M586A/A588Q mutant (solid triangles). (C) Melting curves for native (solid diamonds) and the mutant (solid triangles) proteins measured by monitoring absorbance at 222 nm against increasing time and temperature (2°C increases in temperature every 180 s from 29 to 47°C). All spectra were obtained at room temperature at 0.1 mg/ml in 50 mM phosphate-acetate buffer using a Jobin-Yvon CD6 spectropolarimeter, and are an average of at least two scans after substraction of the spectra of the buffer.
