Article
- The EMBO Journal (2008) 27, 1481 - 1490
- doi:10.1038/emboj.2008.76
Published online: 17 April 2008
Subject Categories:
Hzf regulates adipogenesis through translational control of C/EBP
Hiroyuki Kawagishi1,a, Takeshi Wakoh1,a, Hatsume Uno2, Mitsuo Maruyama1, Ayako Moriya3, Satoru Morikawa3,4, Hideyuki Okano3, Charles J Sherr5,6, Masatoshi Takagi2 and Masataka Sugimoto1
- Department of Mechanism of Aging, National Institute for Longevity Sciences, Aichi, Japan
- Department of Pediatrics and Developmental Biology, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan
- Departmeny of Physiology, Keio University School of Medicine, Tokyo, Japan
- Department of Dentistry and Oral Surgery, Keio University School of Medicine, Tokyo, Japan
- Howard Hughes Medical Institute, St Jude Children's Research Hospital, Memphis, TN, USA
- Department of Genetics and Tumor Cell Biology, St Jude Children's Research Hospital, Memphis, TN, USA
Correspondence to:
Masataka Sugimoto, Department of Mechanism of Aging, National Institute for Longevity Sciences, 36-3 Gengo, Morioka-cho, Obu, Aichi 474-8511, Japan. Tel.: +1 81 562 44 5651 5055; Fax: +1 81 562 46 8461; E-mail: msugimot@nils.go.jp
aThese authors contributed equally to this work
Received 19 September 2007; Accepted 26 March 2008
Abstract
Adipocyte differentiation requires a well-defined programme of gene expression in which the transcription factor C/EBP
(CCAAT/enhancer-binding protein) has a central function. Here, we show that Hzf (haematopoietic zinc-finger), a previously identified p53 transcriptional target, regulates C/EBP expression. Hzf is induced during differentiation of preadipocyte cell lines, and its suppression by short hairpin RNA disrupts adipogenesis. In Hzf's absence, expression of C/EBP is severely impaired because of reduced translation of its mRNA. Hzf physically interacts with the 3' untranslated region of C/EBP mRNA to enhance its translation. Taken together, these findings underscore a critical role of Hzf in the adipogenesis regulatory cascade.
Keywords:
- adipocyte,
- C/EBP,
- Hzf,
- translation
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