Table 1
Lipids revert inert A
amyloid fibrils to neurotoxic protofibrils that affect learning in mice
Ivo Cristiano Martins, Inna Kuperstein, Hannah Wilkinson, Elke Maes, Mieke Vanbrabant, Wim Jonckheere, Patrick Van Gelder, Dieter Hartmann, Rudi D'Hooge, Bart De Strooper, Joost Schymkowitz and Frederic Rousseau
- The EMBO Journal (2008) 27, 224 - 233
- doi:10.1038/sj.emboj.7601953
Published online: 6 December 2007
Back to article| Lipid | Fraction | Neutral red | A11 binding |
|---|---|---|---|
| DMPG | 16.9 | 53.0 6.2 | ++ |
| Cholesterol | 16.9 | 50.53.5 | +++ |
| SM | 16.9 | 43.12.4 | +++ |
| GM1 | 16.9 | 36.12.9 | +++ |
| BTE | 16.9 | 25.24.3 | +++ |
Inert A fibrils (250 mg ml-1, 50  M) were incubated with liposomes enriched in different lipids (left column in the table) (2.5 mg ml-1) overnight while shaking and then centrifuged as indicated previously. The SEC fractionation of the soluble part of A42 fibril/lipid mixtures shows a peak at 16.9 ml. Toxicity of the 16.9 ml peak from the various A42 fibril/lipid emulsions is quantified by neutral red incorporation into hippocampal neuronal cells. A qualitative indication of binding to the oligomer-specific A11 antibody to the 16.9 ml peak from the various A42 fibril/lipid emulsions detected by dot blot is also given. | |||
