Table 1
The post-rigor structure of myosin VI and implications for the recovery stroke
Julie Ménétrey, Paola Llinas, Jérome Cicolari, Gaëlle Squires, Xiaoyan Liu, Anna Li, H Lee Sweeney and Anne Houdusse
- The EMBO Journal (2008) 27, 244 - 252
- doi:10.1038/sj.emboj.7601937
Published online: 29 November 2007
Back to article| Isoform | Cleft orientation angle (deg) between C of Nter residues 120, 160 and | Cleft closure distance (Å), at the indicated location of the cleft, between C of the indicated U50 and L50 kDa residues | ||||
|---|---|---|---|---|---|---|
| U50 kDa residue 418 | U50 kDa residue 598 | Far outer cleft (near actin) 361–536 | Outer cleft (strut) 418–598 | Inner cleft 239–467 | Far inner cleft (switches) 199–463 | |
| Rigor-like state | ||||||
| Myosin VI (2BKH) | 129 | 118 | 18.2 | 8.3 | 11.1 | 10.3 |
| Myosin V (1OE9) | 129 | 120 | 13.2 | 7.2 | 9.6 | 7.9 |
| Squid (2OVK) | 127 | 117 | 13.2 | 7.8 | 9.8 | 7.3 |
| Placo catch (2OS8) | 126 | 115 | 14.3 | 8.4 | 12.9 | 10.4 |
| Post-rigor state | ||||||
| Myosin VI (2VAS) | 148 | 129 | 21.7* | 13.2 | 14.7 | 11.6 |
| Myosin V (1W7J) | 155 | 133 | 20.7 | 14.2 | 16.2 | 12.9 |
| Squid (2OY6) | 150 | 129 | 20.1 | 14.4 | 16.1 | 13.4 |
| Argo catch (2OTG) | 147 | 128 | 20.9 | 13.3 | 16.5 | 13.8 |
| Pre-powerstroke state | ||||||
| Myosin VI (2V26) | 149 | 132 | 22.1 | 11.8 | 12.3 | 8.6 |
| Argo striated (1QVI) | 155 | 134 | 22.9 | 13.3 | 11.8 | 8.8 |
| L50, lower 50-kDa subdomain; Nter, N-terminal; U50, upper 50-kDa subdomain. | ||||||
| Myosin VI residues used for measurements are equivalent to those used for squid from Yang et al (2007). 'Placo' indicates sea scallop myosin II and 'Argo' indicates bay scallop myosin II. (*) This value was measured using a modeled position of residue 361. This residue is indeed absent in the myosin VI post-rigor structure. The model was easily obtained using a superimposition of the U50 kDa subdomain of the myosin VI rigor-like structure (2BKH). | ||||||
