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| Subject Categories: RNA | Proteins |
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| The EMBO Journal
(2007) 26, 2327–2338, doi:10.1038/sj.emboj.7601679 Published online 19 April 2007 |
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| Translation of nonSTOP mRNA is repressed post-initiation in mammalian cells |
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| Nobuyoshi Akimitsu1, Junichi Tanaka2 and Jerry Pelletier3 |
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1 Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Higashi, Tsukuba-shi, Ibaraki, Japan 2 Department of Chemistry, Biology, and Marine Sciences, University of the Ryukyus, Nishihara, Okinawa, Japan 3 Department of Biochemistry, McGill University, McIntyre Medical Sciences Building, Montreal, Quebec, Canada To whom correspondence should be addressed Nobuyoshi Akimitsu, Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1, Higashi, Tsukuba-shi, Ibaraki 305-8566, Japan. Tel.: +81 29 861 6085; Fax: +81 29 861 6095; E-mail: nobu.akimitsu@aist.go.jp Received 22 November 2006; Accepted 15 March 2007; Published online 19 April 2007. |
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| Abstract |
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| We investigated the fate of aberrant mRNAs lacking in-frame termination codons (called nonSTOP mRNA) in mammalian cells. We found that translation of nonSTOP mRNA was considerably repressed although a corresponding reduction of mRNA was not observed. The repression appears to be post-initiation since (i) repressed nonSTOP mRNAs were associated with polysomes, (ii) translation of IRES-initiated and uncapped nonSTOP mRNA were repressed, and (iii) protein production from nonSTOP mRNA associating with polysomes was significantly reduced when used to program an in vitro run-off translation assay. NonSTOP mRNAs distributed into lighter polysome fractions compared to control mRNAs encoding a stop codon, and a significant amount of heterogeneous polypeptides were produced during in vitro translation of nonSTOP RNAs, suggesting premature termination of ribosomes translating nonSTOP mRNA. Moreover, a run-off translation assay using hippuristanol and RNAse protection assays suggested the presence of a ribosome stalled at the 3' end of nonSTOP mRNAs. Taken together, these data indicate that ribosome stalling at the 3' end of nonSTOP mRNAs can block translation by preventing upstream translation events. |
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| Keywords: nonSTOP, ribosome, RNA surveillance, translational repression |
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Top of page MORE ARTICLES LIKE THISThese links to content published by NPG are automatically generated NEWS AND VIEWSNo mercy for messages that mess with the ribosome Nature Structural & Molecular Biology News and Views (01 Apr 2006) Molecular biology RNA lost in translation Nature News and Views (23 Mar 2006) RESEARCHTranslation of nonSTOP mRNA is repressed post-initiation in mammalian cells The EMBO Journal Article The EMBO Journal Article (20 Apr 2005) |
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