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| Subject Categories: Membranes & Transport | Cell Cycle |
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| The EMBO Journal
(2007) 26, 1843–1852, doi:10.1038/sj.emboj.7601621 Published online 8 March 2007 |
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| Cytokinesis in yeast meiosis depends on the regulated removal of Ssp1p from the prospore membrane |
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| Peter Maier, Nicole Rathfelder, Martin G Finkbeiner1, Christof Taxis, Massimiliano Mazza, Sophie Le Panse2, Rosine Haguenauer-Tsapis2 and Michael Knop |
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EMBL, Cell Biology and Biophysics Unit, Heidelberg, Germany To whom correspondence should be addressed Michael Knop, EMBL, Cell Biology and Biophysics Unit, Meyerhofstr. 1, 69117 Heidelberg, Germany. Tel.: +49 6221 387631; Fax: +49 6221 387512; E-mail: knop@embl.de 1 Present address: International Agency for Research on Cancer (IARC), Unit of Gene-Environment Interaction, 150, cours Albert Thomas, 69008 Lyon, France 2 Present address: Institut Jacques Monod-CNRS, Universites Paris VI and VII, 2 Place Jussieu, 75251 PARIS Cedex 05, France Received 6 October 2006; Accepted 26 January 2007; Published online 8 March 2007. |
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| Abstract |
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| Intracellular budding is a developmentally regulated type of cell division common to many fungi and protists. In Saccaromyces cerevisiae, intracellular budding requires the de novo assembly of membranes, the prospore membranes (PSMs) and occurs during spore formation in meiosis. Ssp1p is a sporulation-specific protein that has previously been shown to localize to secretory vesicles and to recruit the leading edge protein coat (LEP coat) proteins to the opening of the PSM. Here, we show that Ssp1p is a multidomain protein with distinct domains important for PI(4,5)P2 binding, binding to secretory vesicles and inhibition of vesicle fusion, interaction with LEP coat components and that it is subject to sumoylation and degradation. We found non-essential roles for Ssp1p on the level of vesicle transport and an essential function of Ssp1p to regulate the opening of the PSM. Together, our results indicate that Ssp1p has a domain architecture that resembles to some extent the septin class of proteins, and that the regulated removal of Ssp1p from the PSM is the major step underlying cytokinesis in yeast sporulation. |
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| Keywords: cytokinesis, intracellular budding, septins, sporulation, yeast |
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Top of page MORE ARTICLES LIKE THISThese links to content published by NPG are automatically generated RESEARCHDefining the specific physiological requirements for c-Myc in T cell development Nature Immunology Article (01 Apr 2001) The EMBO Journal Article (17 Sep 2008) Prospore membrane formation linked to the leading edge protein (LEP) coat assembly The EMBO Journal Article (17 Dec 2001) Nud1p, the yeast homolog of Centriolin, regulates spindle pole body inheritance in meiosis The EMBO Journal Article (23 Aug 2006) |
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