Article
- The EMBO Journal (2007) 26, 1560 - 1568
- doi:10.1038/sj.emboj.7601601
Published online: 22 February 2007
Subject Category:
A novel TBP-associated factor of SL1 functions in RNA polymerase I transcription
Julia J Gorskiab, Shalini Pathaka, Kostya Panov, Taciana Kasciukovic, Tanya Panova, Jackie Russell and Joost CBM Zomerdijk
- Division of Gene Regulation and Expression, College of Life Sciences, Wellcome Trust Biocentre, University of Dundee, Dundee, UK
Correspondence to:
Joost CBM Zomerdijk, Division of Gene Regulation and Expression, College of Life Sciences, Wellcome Trust Biocentre, University of Dundee, Dow Street, Dundee DD1 5EH, UK. Tel.: +44 1382 384242; Fax: +44 1382 348072; E-mail: j.zomerdijk@dundee.ac.uk
aThese authors contributed equally to this work
bPresent address: Cancer Research Centre, Queen's University Belfast, Belfast City Hospital, Belfast, UK
Received 22 August 2006; Accepted 22 January 2007
Abstract
In mammalian RNA polymerase I transcription, SL1, an assembly of TBP and associated factors (TAFs), is essential for preinitiation complex formation at ribosomal RNA gene promoters in vitro. We provide evidence for a novel component of SL1, TAFI41 (MGC5306), which functions in Pol I transcription. TAFI41 resides at the rDNA promoter in the nucleolus and co-purifies and co-immunoprecipitates with SL1. TAFI41 immunodepletion from nuclear extracts dramatically reduces Pol I transcription; addition of SL1 restores the ability of these extracts to support Pol I transcription. In cells, siRNA-mediated decreased expression of TAFI41 leads to loss of SL1 from the rDNA promoter in vivo, with concomitant loss of Pol I from the rDNA and reduced synthesis of the pre-rRNA. Extracts from these cells support reduced levels of Pol I transcription; addition of SL1 to the extracts raises the level of Pol I transcription. These data suggest that TAFI41 is integral to transcriptionally active SL1 and imply a role for SL1, including the TAFI41 subunit, in Pol I recruitment and, therefore, preinitiation complex formation in vivo.
Keywords:
- ribosomal RNA synthesis,
- RNA polymerase I,
- RNA polymerase recruitment,
- SL1,
- TAFI41
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