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| Subject Categories: RNA | Proteins |
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| The EMBO Journal
(2007) 26, 1522–1531, doi:10.1038/sj.emboj.7601598 Published online 22 February 2007 |
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| The adaptor Grb7 links netrin-1 signaling to regulation of mRNA translation |
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| Nien-Pei Tsai, Jing Bi and Li-Na Wei |
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Department of Pharmacology, University of Minnesota Medical School, Minneapolis, MN, USA To whom correspondence should be addressed Li-Na Wei, Department of Pharmacology, University of Minnesota Medical School, 6-120 Jackson Hall, 321 Church St SE, Minneapolis, MN 55455, USA. Tel.: +1 612 6259402; Fax: +1 612 6258408, E-mail: weixx009@umn.edu Received 14 October 2006; Accepted 8 January 2007; Published online 22 February 2007. |
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| Abstract |
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| We previously reported a novel biological activity of Netrin-1 in translational stimulation of kappa opioid receptor (KOR). We now identify Grb7 as a new RNA-binding protein that serves as the molecular adaptor for transmitting Netrin-1 signals, through focal adhesion kinase (FAK), to the translation machinery. Grb7 binds specifically to the first stem loop of kor mRNA 5'-UTR through a new RNA-binding domain located in its amino terminus. Upon binding to its capped, target mRNA, Grb7 blocks the recruitment of eIF4E, rendering mRNA untranslatable. The RNA-binding and translation-repressive activity is reduced by FAK-mediated hyperphosphorylation on two tyrosine residues of its carboxyl terminus. This study reports an adaptor protein Grb7 that transmits the stimulating signals of Netrin-1 to the translational machinery to rapidly regulate mRNA translation. |
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| Keywords: Grb7, Netrin-1, RNA-binding protein, translation |
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Top of page MORE ARTICLES LIKE THISThese links to content published by NPG are automatically generated RESEARCHThe adaptor Grb7 links netrin-1 signaling to regulation of mRNA translation The EMBO Journal Article Regulation of stress granule dynamics by Grb7 and FAK signalling pathway The EMBO Journal Article (05 Mar 2008) Regulation of stress granule dynamics by Grb7 and FAK signalling pathway The EMBO Journal Article |
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