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| Subject Categories: Membranes & Transport | RNA |
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| The EMBO Journal
(2007) 26, 4956–4965, doi:10.1038/sj.emboj.7601901 Published online 22 November 2007 |
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| SAGA and a novel Drosophila export complex anchor efficient transcription and mRNA export to NPC |
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| Maria M Kurshakova1, Alexey N Krasnov1, 2, Daria V Kopytova1, Yulii V Shidlovskii1, Julia V Nikolenko1, Elena N Nabirochkina1, 2, Danièle Spehner3, Patrick Schultz3, Làszlò Tora4 and Sofia G Georgieva1, 2, 5 |
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1 Department of Regulation of Gene Expression, Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia 2 Centre for Medical Studies in Russia, University of Oslo, Moscow, Russia 3 Department of Structural Biology and Genomics, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS UMR, Illkirch Cedex, France 4 Department of Transcription, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS UMR, Illkirch Cedex, France 5 Department of Transcription Factors, Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia To whom correspondence should be addressed Làszlò Tora, Department of Transcription, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS UMR 7104, BP 10142, 67404 Illkirch Cedex, France. Tel.: +33 388 65 34 44; Fax: +33 388 65 32 01; E-mail: laszlo@igbmc.u-strasbg.fr Sofia G Georgieva, Department of Regulation of Gene Expression, Institute of Gene Biology, Russian Academy of Sciences, Moscow 119334, Russia. Tel.: +7 495 135 9731; Fax: +7 495 135 4105; E-mail: sonjag@molbiol.edu.ru Received 3 May 2007; Accepted 1 October 2007; Published online 22 November 2007. |
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| Abstract |
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| SAGA/TFTC-type multiprotein complexes play important roles in the regulation of transcription. We have investigated the importance of the nuclear positioning of a gene, its transcription and the consequent export of the nascent mRNA. We show that E(y)2 is a subunit of the SAGA/TFTC-type histone acetyl transferase complex in Drosophila and that E(y)2 concentrates at the nuclear periphery. We demonstrate an interaction between E(y)2 and the nuclear pore complex (NPC) and show that SAGA/TFTC also contacts the NPC at the nuclear periphery. E(y)2 forms also a complex with X-linked male sterile 2 (Xmas-2) to regulate mRNA transport both in normal conditions and after heat shock. Importantly, E(y)2 and Xmas-2 knockdown decreases the contact between the heat-shock protein 70 (hsp70) gene loci and the nuclear envelope before and after activation and interferes with transcription. Thus, E(y)2 and Xmas-2 together with SAGA/TFTC function in the anchoring of a subset of transcription sites to the NPCs to achieve efficient transcription and mRNA export. |
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| Keywords: heat shock, nuclear envelope, RNA polymerase II transcription, Sac3, Sus1 |
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Top of page MORE ARTICLES LIKE THISThese links to content published by NPG are automatically generated RESEARCHSAGA and a novel Drosophila export complex anchor efficient transcription and mRNA export to NPC The EMBO Journal Article Yeast Ataxin-7 links histone deubiquitination with gene gating and mRNA export Nature Cell Biology Letter (01 Jun 2008) |
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