|
 |
|
|
| Subject Categories: Signal Transduction | Cellular Metabolism |
|
| The EMBO Journal
(2007) 26, 4824–4830, doi:10.1038/sj.emboj.7601914 Published online 8 November 2007 |
|
| Yeast PAS kinase coordinates glucose partitioning in response to metabolic and cell integrity signaling |
|
|
| Julianne H Grose, Tammy L Smith, Hana Sabic and Jared Rutter |
|
|
Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT, USA To whom correspondence should be addressed Jared Rutter, Department of Biochemistry, University of Utah School of Medicine, 15 N Medical Drive East, Salt Lake City, UT 84112, USA. Tel.: +1 801 581 3340; Fax: +1 801 581 7959; E-mail: rutter@biochem.utah.edu Received 22 May 2007; Accepted 17 October 2007; Published online 8 November 2007. |
|
|
|
| Abstract |
|
| PAS kinase is an evolutionarily conserved serine/threonine protein kinase. Mammalian PAS kinase is activated under nutrient replete conditions and is important for controlling metabolic rate and energy homeostasis. In yeast, PAS kinase acts to increase the synthesis of structural carbohydrate at the expense of storage carbohydrates through phosphorylation of the enzyme UDP-glucose pyrophosphorylase. We have identified two pathways that activate yeast PAS kinase; one is responsive to nutrient conditions while the other is responsive to cell integrity stress. These pathways differentially activate the two PAS kinase proteins in Saccharomyces cerevisiae, Psk1 and Psk2, with Psk1 alone responding to activation by nonfermentative carbon sources. We demonstrate that, in addition to transcriptional effects, both of these pathways post-translationally activate PAS kinase via its regulatory N-terminus. As a whole, this system acts to coordinate glucose partitioning with alterations in demand due to changes in environmental and nutrient conditions. |
|
| Keywords: cell integrity signaling, glucose metabolism, kinase regulation |
|
|
|
Top of page MORE ARTICLES LIKE THISThese links to content published by NPG are automatically generated RESEARCHThe EMBO Journal Article |
|
|
| |
| |
| |
 | Email link to a friend |
| |
 | Download PDF |
| |
 | Full text |
| |
| |
| |
 | Next article |
| |
 | Previous article |
| |
 | Table of contents |
| |
| |
 | Rights and permissions |
| |
 | Reprints |
| |
| |
| |
 Register for table of contents by email | |
| |
| |
| |