Figure 3

Increase in the abundance of arc-like filaments in cells overexpressing FtsZ. (A) Differential interference contrast (DIC) and fLM overlay image of cells overexpressing (wild-type) FtsZ, showing that FtsZ-YFP accumulates in the most constricted points. Scale bar 4 m. (B) Low magnification cryo-EM view of a similar cell plunge-frozen in vitreous ice across an EM grid after acquisition of the tilt-series. The gray box shows the region segmented in panel (E). For scale, the circular hole in the carbon support film on the EM grid has a diameter of 2 m. (C) 8 nm slice through the tomogram parallel to the grid. Scale bar 100 nm (for panels C and D). (D) 5 nm slice through the tomogram along the dash line in (C). The white arrow points to the arc-like filaments. (E) 3-D segmentation.