Article
- The EMBO Journal (2007) 26, 4467 - 4474
- doi:10.1038/sj.emboj.7601870
Published online: 4 October 2007
Subject Category:
Histone chaperones regulate histone exchange during transcription
Hye-Jin Kim1, Ja-Hwan Seol1, Jeung-Whan Han1, Hong-Duk Youn2 and Eun-Jung Cho1
- College of Pharmacy, Sungkyunkwan University, Suwon, Gyeonggi-do, Republic of Korea
- Department of Biochemistry and Molecular Biology, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea
Correspondence to:
Eun-Jung Cho, College of Pharmacy, Sungkyunkwan University, 300 Chencheon-dong, Jangan-gu, Suwon, Gyeonggi-do 440-746, Republic of Korea. Tel.: +82 31 290 7781; Fax +82 31 292 8800; E-mail: echo@skku.edu
Received 16 March 2007; Accepted 4 September 2007
Abstract
Transcription by RNA polymerase II is accompanied by dynamic changes in chromatin, including the eviction/deposition of nucleosomes or the covalent modification of histone subunits. This study examined the role of the histone H3/H4 chaperones, Asf1 and HIR, in histone mobility during transcription, with particular focus on the histone exchange pathway, using a dual histone expression system. The results showed that the exchange of H3/H4 normally occurs during transcription by the histone chaperones. Both Asf1 and HIR are important for histone deposition but have a different effect on histone exchange. While Asf1 mediated incorporation of external H3/H4 and renewal of pre-existing histones, HIR opposed it. The balance of two opposing activities might be an important mechanism for determining current chromatin states.
Keywords:
- Asf1,
- HIR,
- histone exchange,
- histone H3/H4 chaperone,
- histone mobility
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