Article
- The EMBO Journal (2007) 26, 448 - 458
- doi:10.1038/sj.emboj.7601488
Published online: 4 January 2007
Subject Categories:
Nutrient starvation promotes condensin loading to maintain rDNA stability
Chi Kwan Tsang1, Hong Li1 and XF Steven Zheng1
- Department of Pharmacology, Robert Wood Johnson Medical School, Piscataway, NJ, USA
Correspondence to:
XF Steven Zheng, Department of Pharmacology, Robert Wood Johnson Medical School, Staged Research Building, Room 142, 675 Hoes Lane, Piscataway, NJ 08854, USA. Tel.: +1 732 235 2894; Fax: +1 732 235 2875; E-mail: zhengst@umdnj.edu
Received 28 August 2006; Accepted 14 November 2006
Abstract
Nutrient starvation or rapamycin treatment, through inhibition of target of rapamycin, causes condensation of ribosomal DNA (rDNA) array and nucleolar contraction in budding yeast. Here we report that under such conditions, condensin is rapidly relocated into the nucleolus and loaded to rDNA tandem repeats, which is required for rDNA condensation. Rpd3-dependent histone deacetylation is necessary and sufficient for condensin's relocalization and loading to rDNA array, suggesting that histone modification plays a regulatory role for condensin targeting. Rapamycin independently, yet coordinately, inhibits rDNA transcription and promotes condensin loading to rDNA array. Unexpectedly, we found that inhibition of rDNA transcription in the absence of condensin loading leads to rDNA instability. Our data suggest that enrichment of condensin prevents rDNA instability during nutrient starvation. Together, these observations unravel a novel role for condensin in the maintenance of regional genomic stability.
Keywords:
- condensin,
- extrachromosomal rDNA circle,
- ribosomal DNA,
- target of rapamycin,
- the nucleolus
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