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| Subject Categories: Genome Stability & Dynamics |
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| The EMBO Journal
(2007) 26, 4126–4137, doi:10.1038/sj.emboj.7601815 Published online 30 August 2007 |
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| AtPRD1 is required for meiotic double strand break formation in Arabidopsis thaliana |
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| Arnaud De Muyt1, Daniel Vezon1, Ghislaine Gendrot1, Jean-Luc Gallois1, Rebecca Stevens2 and Mathilde Grelon1 |
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1 Institut Jean-Pierre Bourgin, INRA de Versailles, Station de Génétique et d'Amélioration des Plantes, Versailles, France 2 Unité de Recherche Génétique et Amélioration des Fruits et Légumes, INRA, Montfavet, France To whom correspondence should be addressed Mathilde Grelon, Institut Jean-Pierre Bourgin, INRA de Versailles, Station de Génétique et d'Amélioration des Plantes, UR-254, Route de St-Cyr, Versailles, 78026 France. Tel.: +33 1 30 83 33 08; Fax: +33 1 30 83 33 19; E-mail: grelon@versailles.inra.fr Received 8 February 2007; Accepted 6 July 2007; Published online 30 August 2007. |
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| Abstract |
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| The initiation of meiotic recombination by the formation of DNA double-strand breaks (DSBs) catalysed by the Spo11 protein is strongly evolutionary conserved. In Saccharomyces cerevisiae, Spo11 requires nine other proteins for meiotic DSB formation, but, unlike Spo11, few of these proteins seem to be conserved across kingdoms. In order to investigate this recombination step in higher eukaryotes, we have isolated a new gene, AtPRD1, whose mutation affects meiosis in Arabidopsis thaliana. In Atprd1 mutants, meiotic recombination rates fall dramatically, early recombination markers (e.g., DMC1 foci) are absent, but meiosis progresses until achiasmatic univalents are formed. Besides, Atprd1 mutants suppress DSB repair defects of a large range of meiotic mutants, showing that AtPRD1 is involved in meiotic recombination and is required for meiotic DSB formation. Furthermore, we showed that AtPRD1 and AtSPO11-1 interact in a yeast two-hybrid assay, suggesting that AtPRD1 could be a partner of AtSPO11-1. Moreover, our study reveals similarity between AtPRD1 and the mammalian protein Mei1, suggesting that AtPRD1 could be a Mei1 functional homologue. |
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| Keywords: Arabidopsis, crossover, DSB, meiosis, recombination |
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Top of page MORE ARTICLES LIKE THISThese links to content published by NPG are automatically generated NEWS AND VIEWSNature Genetics News and Views (01 Mar 2001) Nature News and Views (15 Jan 1976) RESEARCHAtPRD1 is required for meiotic double strand break formation in Arabidopsis thaliana The EMBO Journal Article AtSPO11-1 is necessary for efficient meiotic recombination in plants The EMBO Journal Article (01 Feb 2001) |
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