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| Subject Categories: Signal Transduction | Plant Biology |
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| The EMBO Journal
(2007) 26, 3923–3935, doi:10.1038/sj.emboj.7601826 Published online 9 August 2007 |
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| LysM domains mediate lipochitin–oligosaccharide recognition and Nfr genes extend the symbiotic host range |
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| Simona Radutoiu1, 3, Lene H Madsen1, 3, Esben B Madsen1, Anna Jurkiewicz1, Eigo Fukai1, 4, Esben M H Quistgaard1, Anita S Albrektsen1, Euan K James2, Søren Thirup1 and Jens Stougaard1 |
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1 Laboratory of Gene Expression, Department of Molecular Biology, University of Aarhus, Aarhus, Denmark 2 School of Life Sciences, University of Dundee, Dundee, UK To whom correspondence should be addressed Jens Stougaard, Laboratory of Gene Expression, Department of Molecular Biology, University of Aarhus, Gustav Wieds Vej 10, 8000 Aarhus C, Denmark. Tel.: +45 89 42 50 11; Fax: +45 86 20 12 22; E-mail: stougaard@mb.au.dk 4 These authors contributed equally to this work 3 Present address: Plant Functional Genomics Laboratory, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan Received 3 April 2007; Accepted 18 July 2007; Published online 9 August 2007. |
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| Abstract |
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| Legume–Rhizobium symbiosis is an example of selective cell recognition controlled by host/non-host determinants. Individual bacterial strains have a distinct host range enabling nodulation of a limited set of legume species and vice versa. We show here that expression of Lotus japonicus Nfr1 and Nfr5 Nod-factor receptor genes in Medicago truncatula and L. filicaulis, extends their host range to include bacterial strains, Mesorhizobium loti or DZL, normally infecting L. japonicus. As a result, the symbiotic program is induced, nodules develop and infection threads are formed. Using L. japonicus mutants and domain swaps between L. japonicus and L. filicaulis NFR1 and NFR5, we further demonstrate that LysM domains of the NFR1 and NFR5 receptors mediate perception of the bacterial Nod-factor signal and that recognition depends on the structure of the lipochitin–oligosaccharide Nod-factor. We show that a single amino-acid variation in the LysM2 domain of NFR5 changes recognition of the Nod-factor synthesized by the DZL strain and suggests a possible binding site for bacterial lipochitin–oligosaccharide signal molecules. |
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| Keywords: host range, lipochitin–oligosaccharides, LysM, receptor, symbiosis |
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Top of page MORE ARTICLES LIKE THISThese links to content published by NPG are automatically generated NEWS AND VIEWSPlant biology Locks, keys and symbioses Nature News and Views (09 Oct 2003) Plant?microbe interactions A receptor in symbiotic dialogue Nature News and Views (27 Jun 2002) RESEARCHPlant recognition of symbiotic bacteria requires two LysM receptor-like kinases Nature Article (09 Oct 2003) A receptor kinase gene of the LysM type is involved in legumeperception of rhizobial signals Nature Letters to Editor (09 Oct 2003) |
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