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| Subject Categories: Genome Stability & Dynamics | Microbiology & Pathogens |
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| The EMBO Journal
(2007) 26, 3124–3131, doi:10.1038/sj.emboj.7601747 Published online 7 June 2007 |
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| The two chromosomes of Vibrio cholerae are initiated at different time points in the cell cycle |
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| Tue Rasmussen1, Rasmus Bugge Jensen2 and Ole Skovgaard |
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Department of Science, Systems and Models, Roskilde University, Roskilde, Denmark To whom correspondence should be addressed Ole Skovgaard, Department of Science, Systems and Models, 18-1, Roskilde University, Universitetsvej 1, Roskilde 4000, Denmark. Tel.: +45 4674 2405; Fax: +45 4674 3011; E-mail: olesk@ruc.dk 1 Present address: Department of Pharmaceutics and Analytical Chemistry, University of Copenhagen, Universitetsparken 2, 2100 København Ø, Denmark 2 Present address: LEO Pharma, Industriparken 55, 2750 Ballerup, Denmark Received 2 March 2007; Accepted 16 May 2007; Published online 7 June 2007. |
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| Abstract |
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| The bacterium Vibrio cholerae, the cause of the diarrhoeal disease cholera, has its genome divided between two chromosomes, a feature uncommon for bacteria. The two chromosomes are of different sizes and different initiator molecules control their replication independently. Using novel methods for analysing flow cytometry data and marker frequency analysis, we show that the small chromosome II is replicated late in the C period of the cell cycle, where most of chromosome I has been replicated. Owing to the delay in initiation of chromosome II, the two chromosomes terminate replication at approximately the same time and the average number of replication origins per cell is higher for chromosome I than for chromosome II. Analysis of cell-cycle parameters shows that chromosome replication and segregation is exceptionally fast in V. cholerae. The divided genome and delayed replication of chromosome II may reduce the metabolic burden and complexity of chromosome replication by postponing DNA synthesis to the last part of the cell cycle and reducing the need for overlapping replication cycles during rapid proliferation. |
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| Keywords: C period, divided genomes, flow cytometry, initiation of DNA replication, replication time |
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Top of page MORE ARTICLES LIKE THISThese links to content published by NPG are automatically generated RESEARCHThe two chromosomes of Vibrio cholerae are initiated at different time points in the cell cycle The EMBO Journal Article The EMBO Journal Article (01 Mar 2001) The EMBO Journal Article (02 Jan 2003) |
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