|
 |
|
|
| Subject Categories: Development | Differentiation & Death |
|
| The EMBO Journal
(2007) 26, 184–196, doi:10.1038/sj.emboj.7601480 Published online 7 December 2006 |
|
| Characterization of GATA-1+ hemangioblastic cells in the mouse embryo |
|
|
| Tomomasa Yokomizo1, Satoru Takahashi1, Naomi Mochizuki1, Takashi Kuroha1, Masatsugu Ema1, Asami Wakamatsu1, Ritsuko Shimizu1, Osamu Ohneda1, 2, Motomi Osato3, Hitoshi Okada4, Toshihisa Komori5, Minetaro Ogawa6, Shin-Ichi Nishikawa7, Yoshiaki Ito3 and Masayuki Yamamoto1, 2 |
|
|
1 Institute of Basic Medical Sciences and Center for TARA, University of Tsukuba, Tsukuba, Japan 2 JST-ERATO Environmental Response Project, University of Tsukuba, Tsukuba, Japan 3 Institute of Molecular and Cell Biology and Oncology Research Institute, Proteos, Singapore, Singapore 4 Cancer Institute, Kami-ikebukuro, Toshima-ku, Tokyo, Japan 5 Division of Cell Biology, Department of Developmental and Reconstructive Medicine, Nagasaki University Graduate School of Biomedical Sciences, Sakamoto, Nagasaki, Japan 6 Department of Cell Differentiation, Institute of Molecular Embryology and Genetics, Kumamoto University, Minatojima-minamicho, Chuo-ku, Kobe, Japan 7 Riken Center for Developmental Biology, Minatojima-minamicho, Chuo-ku, Kobe, Japan To whom correspondence should be addressed Satoru Takahashi, Institute of Basic Medical Sciences and Center for TARA, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba 305-8575, Japan. Tel.: +81 29 853 7516; Fax: +81 29 853 6965; E-mail: satoruta@md.tsukuba.ac.jp Masayuki Yamamoto, Institute of Basic Medical Sciences and Center for TARA, University of Tsukuba, Ibaraki, 1-1-1 Tennoudai, Tsukuba 305-8575, Japan. Tel.: +81 29 853 6158; Fax: +81 29 853 7318; E-mail: masi@tara.tsukuba.ac.jp Received 21 March 2006; Accepted 7 November 2006; Published online 7 December 2006. |
|
|
|
| Abstract |
|
| Hemangioblasts are thought to be one of the sources of hematopoietic progenitors, yet little is known about their localization and fate in the mouse embryo. We show here that a subset of cells co-expressing the hematopoietic marker GATA-1 and the endothelial marker VE-cadherin localize on the yolk sac blood islands at embryonic day 7.5. Clonal analysis demonstrated that GATA-1+ cells isolated from E7.0–7.5 embryos include a common precursor for hematopoietic and endothelial cells. Moreover, this precursor possesses primitive and definitive hematopoietic bipotential. By using a transgenic complementation rescue approach, GATA-1+ cell-derived progenitors were selectively restored in Runx1-deficient mice. In the rescued mice, definitive erythropoiesis was recovered but the rescued progenitors did not display multilineage hematopoiesis or intra-aortic hematopoietic clusters. These results provide evidence of the presence of GATA-1+ hemangioblastic cells in the extra-embryonic region and also their functional contribution to hematopoiesis in the embryo. |
|
| Keywords: GATA-1, hemangioblast, rescue, Runx1, VE-cadherin |
|
|
|
Top of page MORE ARTICLES LIKE THISThese links to content published by NPG are automatically generated RESEARCHJournal of Cerebral Blood Flow & Metabolism Original Article Journal of Cerebral Blood Flow & Metabolism Original Article Journal of Cerebral Blood Flow & Metabolism Original Article Circumventing tolerance to a human MDM2-derived tumor antigen by TCR gene transfer Nature Immunology Article (01 Oct 2001) |
|
|
| |
| |
| |
 | Email link to a friend |
| |
 | Download PDF |
| |
 | Full text |
| |
| |
| |
 | Next article |
| |
 | Previous article |
| |
 | Table of contents |
| |
| |
 | Rights and permissions |
| |
 | Reprints |
| |
| |
| |
 Register for table of contents by email | |
| |
| |
| |
