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| Subject Categories:
Development
| Differentiation & Death
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The EMBO Journal
(2007) 26, 184–196, doi:10.1038/sj.emboj.7601480 Published online 7 December 2006
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| Characterization of GATA-1+ hemangioblastic cells in the mouse embryo |
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Tomomasa Yokomizo1, Satoru Takahashi1, Naomi Mochizuki1, Takashi Kuroha1, Masatsugu Ema1, Asami Wakamatsu1, Ritsuko Shimizu1, Osamu Ohneda1, 2, Motomi Osato3, Hitoshi Okada4, Toshihisa Komori5, Minetaro Ogawa6, Shin-Ichi Nishikawa7, Yoshiaki Ito3 and Masayuki Yamamoto1, 2
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1 Institute of Basic Medical Sciences and Center for TARA, University of Tsukuba, Tsukuba, Japan
2 JST-ERATO Environmental Response Project, University of Tsukuba, Tsukuba, Japan
3 Institute of Molecular and Cell Biology and Oncology Research Institute, Proteos, Singapore, Singapore
4 Cancer Institute, Kami-ikebukuro, Toshima-ku, Tokyo, Japan
5 Division of Cell Biology, Department of Developmental and Reconstructive Medicine, Nagasaki University Graduate School of Biomedical Sciences, Sakamoto, Nagasaki, Japan
6 Department of Cell Differentiation, Institute of Molecular Embryology and Genetics, Kumamoto University, Minatojima-minamicho, Chuo-ku, Kobe, Japan
7 Riken Center for Developmental Biology, Minatojima-minamicho, Chuo-ku, Kobe, Japan
To whom correspondence should be addressed
Satoru Takahashi, Institute of Basic Medical Sciences and Center for TARA, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba 305-8575, Japan. Tel.: +81 29 853 7516; Fax: +81 29 853 6965; E-mail: satoruta@md.tsukuba.ac.jp Masayuki Yamamoto, Institute of Basic Medical Sciences and Center for TARA, University of Tsukuba, Ibaraki, 1-1-1 Tennoudai, Tsukuba 305-8575, Japan. Tel.: +81 29 853 6158; Fax: +81 29 853 7318; E-mail: masi@tara.tsukuba.ac.jp
Received 21 March 2006; Accepted 7 November 2006; Published online 7 December 2006.
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| Abstract |
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| Hemangioblasts are thought to be one of the sources of hematopoietic progenitors, yet little is known about their localization and fate in the mouse embryo. We show here that a subset of cells co-expressing the hematopoietic marker GATA-1 and the endothelial marker VE-cadherin localize on the yolk sac blood islands at embryonic day 7.5. Clonal analysis demonstrated that GATA-1+ cells isolated from E7.0–7.5 embryos include a common precursor for hematopoietic and endothelial cells. Moreover, this precursor possesses primitive and definitive hematopoietic bipotential. By using a transgenic complementation rescue approach, GATA-1+ cell-derived progenitors were selectively restored in Runx1-deficient mice. In the rescued mice, definitive erythropoiesis was recovered but the rescued progenitors did not display multilineage hematopoiesis or intra-aortic hematopoietic clusters. These results provide evidence of the presence of GATA-1+ hemangioblastic cells in the extra-embryonic region and also their functional contribution to hematopoiesis in the embryo. |
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| Keywords: GATA-1, hemangioblast, rescue, Runx1, VE-cadherin |
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