Figure 4

Gel assays for KaiC hexamerization and KaiA–KaiC complex formation with wild type and a C-terminal deletion mutant of KaiC (25CThKaiC). (A) Native gel-electrophoretic assay for the formation of the complex between full-length ThKaiA dimer and ThKaiC hexamer. A '+' indicates a 10 pM concentration of KaiA dimer and a 15 pM concentration of KaiC hexamer. Additional '+' signs indicate increases by 10 and 15 pM in the concentrations of the KaiA dimers and KaiC hexamers, respectively. A '-' indicates absence of protein. (B) Native gel-electrophoretic assay for the formation of the complex between full-length SeKaiA dimer and SeKaiC hexamer. See (A) for explanation regarding protein concentrations. (C) Native gel-electrophoretic assay showing absence of complex formation in mixtures of full-length ThKaiA dimer and 25CThKaiC hexamer. See (A) for explanation regarding protein concentrations.