Figure 4
Cockayne syndrome B protein regulates the transcriptional program after UV irradiation
Luca Proietti-De-Santis, Pascal Drané and Jean-Marc Egly
- The EMBO Journal (2006) 25, 1915 - 1923
- doi:10.1038/sj.emboj.7601071
Published online: 6 April 2006
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ChIP analysis on the CYP24 promoter in WT and CS8PV cells. Soluble chromatin was prepared from either UV irradiated (left panels) or unirradiated (right panels) cells co-treated with vit-D (10-7 M) for the indicated time and immunoprecipitated with antibodies against RNA pol II (A, B), VDR (C, D), CSB (E, F) or the acetylated histone H4 (G, H). The genomic DNAs were analyzed by quantitative PCR. Western blot analyzes (I, J) of VDR protein from nuclear extracts of WT and CSB cells, either UV irradiated or unirradiated, at different time points after vit-D treatment.
