Article
- The EMBO Journal (2006) 25, 1906 - 1914
- doi:10.1038/sj.emboj.7601068
Published online: 20 April 2006
Subject Category:
SUMO conjugation attenuates the activity of the gypsy chromatin insulator
Maya Capelson and Victor G Corces
- Department of Biology, Johns Hopkins University, Baltimore, MD, USA
Correspondence to:
Victor G Corces, Department of Biology, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA. Tel.: +1 410 516 8749; Fax: +1 410 516 4990; E-mail: corces@jhu.edu
Received 19 December 2005; Accepted 8 March 2006
Abstract
Chromatin insulators have been implicated in the establishment of independent gene expression domains and in the nuclear organization of chromatin. Post-translational modification of proteins by Small Ubiquitin-like Modifier (SUMO) has been reported to regulate their activity and subnuclear localization. We present evidence suggesting that two protein components of the gypsy chromatin insulator of Dorsophila melanogaster, Mod(mdg4)2.2 and CP190, are sumoylated, and that SUMO is associated with a subset of genomic insulator sites. Disruption of the SUMO conjugation pathway improves the enhancer-blocking function of a partially active insulator, indicating that SUMO modification acts to regulate negatively the activity of the gypsy insulator. Sumoylation does not affect the ability of CP190 and Mod(mdg4)2.2 to bind chromatin, but instead appears to regulate the nuclear organization of gypsy insulator complexes. The results suggest that long-range interactions of insulator proteins are inhibited by sumoylation and that the establishment of chromatin domains can be regulated by SUMO conjugation.
Keywords:
- chromatin,
- insulator,
- nuclear organization,
- SUMO,
- transcription
