|
 |
|
|
| Subject Categories: Chromatin & Transcription | Differentiation & Death |
|
| The EMBO Journal
(2006) 25, 1680–1689, doi:10.1038/sj.emboj.7601066 Published online 6 April 2006 |
|
| Tip60 and p400 are both required for UV-induced apoptosis but play antagonistic roles in cell cycle progression |
|
|
| Sandrine Tyteca, Marie Vandromme, Gaëlle Legube, Martine Chevillard-Briet and Didier Trouche |
|
|
Laboratoire de Biologie Moléculaire Eucaryote (LBME), UMR 5099 CNRS/Université Paul Sabatier, Institut d'Exploration Fonctionnelle des Génomes (IEFG), Toulouse, France To whom correspondence should be addressed Didier Trouche, Université Paul Sabatier, CNRS-LBME, Bat IBCG, 118, Route de Narbonne, 31062 Toulouse Cedex 9, France. Tel.: +33 5 61 33 59 15; Fax: +33 5 61 33 58 86; E-mail: trouche@ibcg.biotoul.fr Received 24 October 2005; Accepted 7 March 2006; Published online 6 April 2006. |
|
|
|
| Abstract |
|
| The histone acetyl transferase Tip60 (HTATIP) belongs to a multimolecular complex involved in the cellular response to DNA damage. Tip60 participates in cell cycle arrest following DNA damage by allowing p53 to activate p21CIP (p21) expression. We show here that Tip60 and the E1A-associated p400 protein (EP400), which belongs to the Tip60 complex, are also required for DNA damage-induced apoptosis. Tip60 favours the expression of some proapoptotic p53 target genes most likely through the stimulation of p53 DNA binding activity. In contrast, p400 represses p21 expression in unstressed cells, thereby allowing cell cycle progression and DNA damage-induced apoptosis. Tip60 and p400 have thus opposite effects on p21 expression in the absence of DNA damage. We further found that this antagonism relies on the inhibition of Tip60 function by p400, a property that is abolished following DNA damage. Therefore, taken together, our results indicate that Tip60 and p400 play distinct roles in DNA damage-induced apoptosis and underline the importance of the Tip60 complex and its regulation in the proper control of cell fate. |
|
| Keywords: apoptosis, cell cycle, DNA damage, E1A-associated p400, Tip60 complex |
|
|
|
Top of page MORE ARTICLES LIKE THISThese links to content published by NPG are automatically generated NEWS AND VIEWSDeubiquitinating PCNA: a downside to DNA damage tolerance Nature Cell Biology News and Views (01 Apr 2006) RESEARCHA GTP-binding adapter protein couples TRAIL receptors to apoptosis-inducing proteins Nature Immunology Article (01 Jun 2001) Circumventing tolerance to a human MDM2-derived tumor antigen by TCR gene transfer Nature Immunology Article (01 Oct 2001) Nature Biotechnology Research (01 Nov 2003) |
|
|
| |
| |
| |
 | Email link to a friend |
| |
 | Download PDF |
| |
 | Full text |
| |
| |
| |
 | Next article |
| |
 | Previous article |
| |
 | Table of contents |
| |
| |
 | Rights and permissions |
| |
 | Reprints |
| |
| |
| |
 Register for table of contents by email | |
| |
| |
| |
