Article
- The EMBO Journal (2006) 25, 1669 - 1679
- doi:10.1038/sj.emboj.7601046
Published online: 6 April 2006
Subject Category:
Nucleolin is a histone chaperone with FACT-like activity and assists remodeling of nucleosomes
Dimitar Angelov1,2, Vladimir A Bondarenko3, Sébastien Almagro1,2, Hervé Menoni1,2, Fabien Mongélard1,2, Fabienne Hans4, Flore Mietton4, Vasily M Studitsky3, Ali Hamiche5, Stefan Dimitrov2,4 and Philippe Bouvet1,2
- Ecole Normale Supérieure de Lyon, CNRS-UMR 5161/INRA 1237/IFR128 Biosciences, Lyon-Gerland, France
- Laboratoire Joliot-Curie, Lyon, France
- Department of Pharmacology, University of Medicine and Dentistry of New Jersey, Piscataway, NJ, USA
- Institut Albert Bonniot, INSERM U309, La Tronche Cedex, France
- Institut André Lwoff, CNRS UPR 9079, Villejuif, France
Correspondence to:
Stefan Dimitrov, Institut Albert Bonniot, INSERM U309, La Tronche, France. Tel.: +33 4 76 54 94 73; Fax: +33 4 76 54 95 95; E-mail: stefan.dimitrov@ujf-grenoble.fr
Philippe Bouvet, Ecole Normale Supérieure de Lyon, CNRS-UMR 5161/INRA 1237/IFR128, Biosciences Lyon-Gerland, France. Tel./Fax: +33 4 72 72 80 16; E-mail: pbouvet@ens-lyon.fr
Received 8 December 2005; Accepted 21 February 2006
Abstract
Remodeling machines play an essential role in the control of gene expression, but how their activity is regulated is not known. Here we report that the nuclear protein nucleolin possesses a histone chaperone activity and that this factor greatly enhances the activity of the chromatin remodeling machineries SWI/SNF and ACF. Interestingly, nucleolin is able to induce the remodeling by SWI/SNF of macroH2A, but not of H2ABbd nucleosomes, which are otherwise resistant to remodeling. This new histone chaperone promotes the destabilization of the histone octamer, helping the dissociation of a H2A–H2B dimer, and stimulates the SWI/SNF-mediated transfer of H2A–H2B dimers. Furthermore, nucleolin facilitates transcription through the nucleosome, which is reminiscent of the activity of the FACT complex. This work defines new functions for histone chaperones in chromatin remodeling and regulation of transcription and explains how nucleolin could act on transcription.
Keywords:
- macroH2A and H2ABbd histone variants,
- nucleolin,
- nucleosome,
- remodeling,
- transcription
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