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| Subject Categories: Signal Transduction | Immunology |
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| The EMBO Journal
(2006) 25, 774–784, doi:10.1038/sj.emboj.7600978 Published online 9 February 2006 |
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Recruitment and activation of PLC 1 in T cells: a new insight into old domains |
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| Alex Braiman, Mira Barda-Saad, Connie L Sommers and Lawrence E Samelson |
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Laboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA To whom correspondence should be addressed Lawrence E Samelson, Laboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Building 37, Room 2066, Bethesda, MD 20892-4254, USA. Tel.: +1 301 496 9683; Fax: +1 301 496 8479; E-mail: samelson@helix.nih.gov Received 25 August 2005; Accepted 9 January 2006; Published online 9 February 2006. |
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| Abstract |
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| Engagement of the T-cell antigen receptor leads to recruitment of phospholipase C1 (PLC1) to the LAT-nucleated signaling complex and to PLC1 activation in a tyrosine phosphorylation-dependent manner. The mechanism of PLC1 recruitment and the role of PLC1 Src homology (SH) domains in this process remain incompletely understood. Using a combination of biochemical methods and real-time fluorescent imaging, we show here that the N-terminal SH2 domain of PLC1 is necessary but not sufficient for its recruitment. Either the SH3 or C-terminal SH2 domain of PLC1, with the participation of Vav1, c-Cbl and Slp76, are required to stabilize PLC1 recruitment. All three PLC1 SH domains are required for phosphorylation of PLC1 Y783, which is critical for enzyme activation. These novel findings entailed revision of the currently accepted model of PLC1 recruitment and activation in T lymphocytes. |
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| Keywords: calcium, confocal microscopy, FRET, protein–protein interaction, TCR |
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Top of page MORE ARTICLES LIKE THISThese links to content published by NPG are automatically generated RESEARCHThe EMBO Journal Article (01 Apr 1999) A PAK1?PIX?PKL complex is activated by the T-cell receptor independent of Nck, Slp-76 and LAT The EMBO Journal Article (01 Feb 2001) Nature Immunology Article (01 Jun 2005) |
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