Article
- The EMBO Journal (2006) 25, 846 - 856
- doi:10.1038/sj.emboj.7600975
Published online: 9 February 2006
Subject Category:
Subtelomeric proteins negatively regulate telomere elongation in budding yeast
Anne-Sophie Berthiau1, Krassimir Yankulov1,2, Amadou Bah1,3, Emmanuelle Revardel1,a, Pierre Luciano4, Raymund J Wellinger3, Vincent Géli4 and Eric Gilson1
- Laboratoire de Biologie Moléculaire de la Cellule de l'Ecole Normale Supérieure de Lyon, CNRS UMR5161, IFR 128, Lyon, France
- Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada
- Department of Microbiology and Infectious Diseases, Faculty of Medicine, Universite de Sherbrooke 3001, Sherbrooke, Quebec, Canada
- Laboratoire d'Instabilité Génétique et Cancérogenèse (IGC), CNRS, Marseille, France
Correspondence to:
Eric Gilson, Laboratoire de Biologie Moléculaire et Cellulaire, Ecole Normale Supérieure, UMR5161 CNRS/ENSL, 46 Allee d'italie, 69364 Lyon Cedex 07, France. Tel.: +33 472 728 453; Fax: +33 472 728 686; E-mail: eric.gilson@ens-lyon.fr
aPresent address: Université Bordeaux 1, Avenue des Facultés, 33405 Talence Cedex, France
Received 12 October 2005; Accepted 9 January 2006
Abstract
The Tbf1 and Reb1 proteins are present in yeast subtelomeric regions. We establish in this work that they inhibit telomerase-dependent lengthening of telomere. For example, tethering the N-terminal domain of Tbf1 and Reb1 in a subtelomeric region shortens that telomere proportionally to the number of domains bound. We further identified a 90 amino-acid long sequence within the N-terminal domain of Tbf1 that is necessary but not sufficient for its length regulation properties. The role of the subtelomeric factors in telomere length regulation is antagonized by TEL1 and does not correlate with a global telomere derepression. We show that the absence of TEL1 induces an alteration in the structure of telomeric chromatin, as defined biochemically by an increased susceptibility to nucleases and a greater heterogeneity of products. We propose that the absence of TEL1 modifies the organization of the telomeres, which allows Tbf1 and Reb1 to cis-inhibit telomerase. The involvement of subtelomeric factors in telomere length regulation provides a possible mechanism for the chromosome-specific length setting observed at yeast and human telomeres.
Keywords:
- Reb1,
- subtelomeric repeat,
- Tbf1,
- telomerase,
- telomere
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