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| Subject Categories: Chromatin & Transcription | Immunology |
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| The EMBO Journal
(2006) 25, 811–821, doi:10.1038/sj.emboj.7600966 Published online 26 January 2006 |
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| Transcription regulation from a TATA and INR-less promoter: spatial segregation of promoter function |
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| Anuja A George, Manish Sharma, Badri N Singh, Naresh C Sahoo and Kanury VS Rao |
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Immunology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India To whom correspondence should be addressed Kanury VS Rao, Immunology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India. Tel.: +91 11 2617 6680; Fax: +91 11 267 5114; E-mail: kanury.rao@gmail.com Received 18 October 2005; Accepted 23 December 2005; Published online 26 January 2006. |
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| Abstract |
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The mode of regulation of class II genes that lack the known core promoter elements is presently unclear. Here, we studied one such example, the murine CD80 gene. An unusual mechanism was revealed wherein the pre-initiation complex (PIC) first assembled on an upstream, NF- B enhancer element. Notably, this assembly occurred independent of contributions from the core promoter domain, and resulted in a PIC that was competent for transcription initiation. Positioning was subsequently achieved by exploiting the intrinsic architecture of the promoter, by virtue of which the tethered PIC was spatially juxtaposed with the transcription initiation site. Bridging interactions then ensued, through protein–protein contacts, which then enabled the elongation phase of CD80 transcription. |
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| Keywords: activation, CD80, transcription |
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Top of page MORE ARTICLES LIKE THISThese links to content published by NPG are automatically generated NEWS AND VIEWSRepairing nucleosomes during transcription Nature Structural Biology News and Views (01 Nov 2003) Nature News and Views (11 Aug 1994) RESEARCHThe EMBO Journal Article (15 Jun 2001) SIR repression of a yeast heat shock gene: UAS and TATA footprints persist within heterochromatin The EMBO Journal Article (15 Dec 1999) |
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