Figure 2

Generation of RIM2 KO mice. (A) Structures of the RIM2 wild-type gene (wild-type allele), of the targeting vector used for homologous recombination (targeting construct), and of the mutant RIM2 alleles after homologous recombination before and after further recombination of flp and cre recombinases. In the targeting vector, exon 5 is flanked by loxP sites (black triangles) and the neomycin resistance gene cassette (neo) that is flanked by flp recombination sites (black circles). A diphtheria toxin gene (DT) is included for negative selection. (B) Weights of male RIM2 KO and littermate control mice (N=36, ^*P<0.001). (C) Immunoblots of E18.5 embryonic and adult wild-type, RIM1 KO, RIM2 KO and embryonic DKO whole brain homogenates. (D) -RIM immunoblots of proteins from different brain regions from adult RIM1 KO, RIM2 KO, and wild-type control mice; blots were probed with an antibody that recognizes both RIM1 and RIM2 (abbreviations of brain areas: OB, olfactory bulb; STR, striatum; CTX, cortex; HC, hippocampus; TH, thalamus; MB, midbrain, BS, brain stem; CB, cerebellum; ^*, bands of alternatively spliced RIM2 isoforms of higher molecular weight).