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Figure 4
Excessive avoidance in grk-2 animals counterbalances chemoattraction to NaCl. (A) Chemotaxis to 0.1–100 mM NaCl was impaired in grk-2 animals (P<0.0001). The grk-2 defect could be restored by expressing GRK-2 in the nociceptive neurons in strains grk-2 gjEx601 (P<0.05), gjEx602 (P<0.001) and gjEx639 (P<0.001). (B) grk-2 mutants showed no chemoattraction to 25 mM NaCl, but strong avoidance after pre-exposure to 100 mM NaCl. Expression of GRK-2 in the nociceptive neurons restored chemoattraction in grk-2 gjEx601 animals (P<0.001) and grk-2 gjEx639 animals ((P<0.0001). grk-2 gjEx602 animals showed even better rescue of chemoattraction (P<0.0001), but impaired avoidance after pre-exposure (P<0.0001). Mutation of arr-1 significantly reduced avoidance after pre-exposure (P<0.001), to a similar level as loss of gpa-1. The behaviour of the gpa-1 arr-1 double mutant suggests that both genes function in the same pathway (P>0.05). (C) grk-2 animals showed enhanced avoidance after pre-exposure to NaCl for only 5 min (P<0.001). (D) grk-2 animals showed strong avoidance of 1 M NaCl, which was not affected in grk-2 gjEx601 and gjEx639 animals (P>0.05), but impaired in grk-2 gjEx602 animals (P<0.001).
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