Article
- The EMBO Journal (2006) 25, 387 - 397
- doi:10.1038/sj.emboj.7600933
Published online: 12 January 2006
Subject Category:
Initiation of DNA repair mediated by a stalled RNA polymerase IIO
Jean-Philippe Lainé and Jean-Marc Egly
- Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, Illkirch Cedex, CU Strasbourg, France
Correspondence to:
Jean-Marc Egly, Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, BP 163, 67404 Illkirch Cedex, CU Strasbourg, France. Tel.: +33 388 65 34 47; Fax: +33 388 65 32 01; E-mail: egly@igbmc.u-strasbg.fr
Received 24 October 2005; Accepted 2 December 2005
Abstract
The transcription-coupled repair (TCR) pathway preferentially repairs DNA damage located in the transcribed strand of an active gene. To gain insight into the coupling mechanism between transcription and repair, we have set up an in vitro system in which we isolate an elongating RNA pol IIO, which is stalled in front of a cisplatin adduct. This immobilized RNA pol IIO is used as 'bait' to sequentially recruit TFIIH, XPA, RPA, XPG and XPF repair factors in an ATP-dependent manner. This RNA pol IIO/repair complex allows the ATP-dependent removal of the lesion only in the presence of CSB, while the latter does not promote dual incision in an XPC-dependent nucleotide excision repair reaction. In parallel to the dual incision, the repair factors also allow the partial release of RNA pol IIO. In this 'minimal TCR system', the RNA pol IIO can effectively act as a loading point for all the repair factors required to eliminate a transcription-blocking lesion.
Keywords:
- CSB,
- NER,
- RNA pol II,
- transcription
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