Figure 2
SAGA binds TBP via its Spt8 subunit in competition with DNA: implications for TBP recruitment
Decha Sermwittayawong and Song Tan
- The EMBO Journal (2006) 25, 3791 - 3800
- doi:10.1038/sj.emboj.7601265
Published online: 3 August 2006
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Label transfer photo-crosslinking identifies Spt8 and Ada1 as proximal SAGA subunit targets of TBP. The biotin label on the crosslinked samples was visualized by Western blotting using HRP-conjugated Streptavidin (lanes 1–4) and using anti-Spt8 (lanes 5–8) and anti-Ada1 antibodies (lanes 9–12) after the same blot was stripped and reprobed.
