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The EMBO Journal (2006) 25, 3774–3783, doi:10.1038/sj.emboj.7601263 Published online 3 August 2006 |
| Figure 7 |
| MUC1 oncoprotein blocks nuclear targeting of c-Abl in the apoptotic response to DNA damage |
| Deepak Raina, Rehan Ahmad, Shailendra Kumar, Jian Ren, Kiyotsugu Yoshida, Surender Kharbanda and Donald Kufe |
B
. Intensity of the signals was determined by densitometric scanning and compared to that for untreated cells. (B) Anti-MUC1-N precipitates from HCT116/MUC1 cells treated with CDDP for the indicated times were immunoblotted with anti-c-Abl and anti-MUC1-N. (C) HCT116/vector, HCT116/MUC1 and HCT116/MUC1(Y60F) cells were treated with CDDP for indicated times. Lysates were immunoblotted with anti-PKC
and anti-
-actin. FL: full-length. CF: cleaved fragments. (D) The results from sub-G1 DNA analysis of HCT116/vector, HCT116/MUC1 and HCT116/MUC1(Y60F) cells treated with CDDP for 0 h (open bars), 12 h (solid bars) or 24 h (hatched bars) are expressed as the percentage apoptosis (mean
s.d. of three separate experiments). (E) The results from sub-G1 DNA analysis of HCT116/vector, HCT116/MUC1 and HCT116/MUC1(Y60F) cells left untreated (open bars) or treated with CDDP alone for 12 h (solid bars), STI571 alone for 12 h (shaded bars) or both CDDP and STI571 for 12 h (hatched bars) are expressed as the percentage apoptosis (mean
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