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The EMBO Journal (2006) 25, 3774–3783, doi:10.1038/sj.emboj.7601263 Published online 3 August 2006 Figure 6 MUC1 oncoprotein blocks nuclear targeting of c-Abl in the apoptotic response to DNA damage Deepak Raina, Rehan Ahmad, Shailendra Kumar, Jian Ren, Kiyotsugu Yoshida, Surender Kharbanda and Donald Kufe

Figure 6 MUC1 blocks c-Abl Thr-735 phosphorylation and binding of c-Abl to 14-3-3. (A–C) Lysates from HeLa/vector, HeLa/MUC1 (A), ZR-75-1/vector, ZR-75-1/MUC1siRNA (B), HCT116/vector, HCT116/MUC1 and HCT116/MUC1(Y60F) (C) cells were immunoprecipitated with anti-c-Abl. The precipitates were immunoblotted with anti-phospho-c-Abl-Thr-735 and anti-c-Abl. (D–F) Cytoplasmic fractions of HeLa/vector, HeLa/MUC1 (D), ZR-75-1/vector, ZR-75-1/MUC1siRNA (E), HCT116/vector, HCT116/MUC1 and HCT116/MUC1(Y60F) (F) cells were immunoprecipitated with anti-14-3-3. The precipitates were immunoblotted with anti-c-Abl and anti-14-3-3.

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