View the Current Issue

Article

  • The EMBO Journal (2006) 25, 3774 - 3783
  • doi:10.1038/sj.emboj.7601263

Published online: 3 August 2006

MUC1 oncoprotein blocks nuclear targeting of c-Abl in the apoptotic response to DNA damage

Deepak Rainaa, Rehan Ahmada, Shailendra Kumarb, Jian Ren, Kiyotsugu Yoshidac, Surender Kharbanda and Donald Kufe

  1. Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA, USA

Correspondence to:

Donald Kufe, Dana-Farber Cancer Institute, Harvard Medical School, Binny Street, Boston, MA 02115, USA. Tel.: +1 617 632 3141 Fax: +1 617 632 2934; E-mail: donald_kufe@dfci.harvard.edu

aThese authors contributed equally to this work

bPresent address: Matritech Inc., Newton, MA 02460, USA

cPresent address: Medical Research Institute, Tokyo Medical and Dental University, Tokyo 113-8510, Japan

Received 15 December 2005; Accepted 7 July 2006

The nonreceptor c-Abl tyrosine kinase binds to cytosolic 14-3-3 proteins and is targeted to the nucleus in the apoptotic response to DNA damage. The MUC1 oncoprotein is overexpressed by most human carcinomas and blocks the induction of apoptosis by genotoxic agents. Using human carcinoma cells with gain and loss of MUC1 function, we show that nuclear targeting of c-Abl by DNA damage is abrogated by a MUC1-dependent mechanism. The results demonstrate that c-Abl phosphorylates MUC1 on Tyr-60 and forms a complex with MUC1 by binding of the c-Abl SH2 domain to the pTyr-60 site. Binding of MUC1 to c-Abl attenuates phosphorylation of c-Abl on Thr-735 and the interaction between c-Abl and cytosolic 14-3-3. We also show that expression of MUC1 with a mutation at Tyr-60 (i) disrupts the interaction between MUC1 and c-Abl, (ii) relieves the MUC1-induced block of c-Abl phosphorylation on Thr-735 and binding to 14-3-3, and (iii) attenuates the MUC1 antiapoptotic function. These findings indicate that MUC1 sequesters c-Abl in the cytoplasm and thereby inhibits apoptosis in the response to genotoxic anticancer agents.

  • Keywords:

    • apoptosis,
    • c-Abl,
    • DNA damage,
    • MUC1,
    • 14-3-3