Article
- The EMBO Journal (2006) 25, 184 - 195
- doi:10.1038/sj.emboj.7600906
Published online: 8 December 2005
Subject Categories:
Essential role of Isd11 in mitochondrial iron–sulfur cluster synthesis on Isu scaffold proteins
Nils Wiedemann1, Eugen Urzica2, Bernard Guiard3, Hanne Müller1, Christiane Lohaus4, Helmut E Meyer4, Michael T Ryan5, Chris Meisinger1, Ulrich Mühlenhoff2, Roland Lill2 and Nikolaus Pfanner1
- Institut für Biochemie und Molekularbiologie, Universität Freiburg, Freiburg, Germany
- Institut für Zytobiologie und Zytopathologie, Philipps-Universität Marburg, Marburg, Germany
- Centre de Génétique Moléculaire, CNRS, Gif-sur-Yvette, France
- Medizinisches Proteom-Center, Ruhr-Universität Bochum, Bochum, Germany
- Department of Biochemistry, La Trobe University, Melbourne, Australia
Correspondence to:
Nikolaus Pfanner, Institut für Biochemie und Molekularbiologie, Universität Freiburg, Hermann-Herder-Strasse 7, 79104 Freiburg, Germany. Tel.: +49 761 203 5224; Fax: +49 761 203 5261; E-mail: nikolaus.pfanner@biochemie.uni-freiburg.de
Received 9 August 2005; Accepted 15 November 2005
Abstract
Mitochondria are indispensable for cell viability; however, major mitochondrial functions including citric acid cycle and oxidative phosphorylation are dispensable. Most known essential mitochondrial proteins are involved in preprotein import and assembly, while the only known essential biosynthetic process performed by mitochondria is the biogenesis of iron–sulfur clusters (ISC). The components of the mitochondrial ISC-assembly machinery are derived from the prokaryotic ISC-assembly machinery. We have identified an essential mitochondrial matrix protein, Isd11 (YER048w-a), that is found in eukaryotes only. Isd11 is required for biogenesis of cellular Fe/S proteins and thus is a novel subunit of the mitochondrial ISC-assembly machinery. It forms a complex with the cysteine desulfurase Nfs1 and is required for formation of an Fe/S cluster on the Isu scaffold proteins. We conclude that Isd11 is an indispensable eukaryotic component of the mitochondrial machinery for biogenesis of Fe/S proteins.
Keywords:
- essential cellular functions,
- Fe/S proteins,
- mitochondria,
- Saccharomyces cerevisiae
MORE ARTICLES LIKE THIS
These links to content published by NPG are automatically generated
REVIEWS
Multiple pathways for sorting mitochondrial precursor proteins
EMBO reports Review (01 Jan 2008)
NEWS AND VIEWS
Assembling iron-sulfur clusters in the cytosol
Nature Chemical Biology News and Views (01 May 2007)
Nature Chemical Biology News and Views (01 Jun 2009)
RESEARCH
The Nfs1 interacting protein Isd11 has an essential role in Fe/S cluster biogenesis in mitochondria
The EMBO Journal Article (11 Jan 2006)
The EMBO Journal Article (15 Sep 2003)
