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Figure 7
Induction of Csk in transfected CHO cells slows down cell proliferation only if wt-VE-cadherin is coexpressed. CHO cells expressing inducible wt form of Csk (Csk-wt) or the kinase mutant of Csk (Csk-mt) were cotransfected with wt form of VE-cadherin (VE-cad-wt), or VE-cadherin-Y685F (VE-cad-Y685F) or without VE-cadherin (without VE-cad), and were analyzed for [3H]thymidine incorporation. In each case, two different transfected clones were analyzed. For each clone, [3H]thymidine incorporation of cells not induced for Csk expression was set as 100% (black bar). Measurements were carried out with cells at early confluence, resembling the stage between 72 and 96 h growth in Figure 8. At this stage, [3H]thymidine incorporation of wt-VE-cadherin and mt-VE-cadherin cells in the absence of Csk induction was similar (within a range of 15%, not shown), in agreement with the results in Figure 8.
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