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| Subject Categories: RNA | Microbiology & Pathogens |
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| The EMBO Journal
(2005) 24, 824–835, doi:10.1038/sj.emboj.7600572 Published online 27 January 2005 |
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| Staphylococcus aureus RNAIII and the endoribonuclease III coordinately regulate spa gene expression |
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| Eric Huntzinger1, 5, Sandrine Boisset2, 5, Cosmin Saveanu3, Yvonne Benito2, Thomas Geissmann1, Abdelkader Namane4, Gérard Lina2, Jerome Etienne2, Bernard Ehresmann1, Chantal Ehresmann1, Alain Jacquier3, François Vandenesch2 and Pascale Romby1 |
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1 UPR 9002 du CNRS, Institut de Biologie Moléculaire et Cellulaire, Strasbourg Cedex, France 2 Faculté de Médecine Laennec, National Reference Center for Staphylococci, INSERM E0230, Lyon Cedex, France 3 URA2171-CNRS-Génétique des Interactions Macromoléculaires, Paris Cedex, France 4 Plateforme protéomique, Institut Pasteur, Paris Cedex, France To whom correspondence should be addressed Pascale Romby, UPR 9002 du CNRS, Institut de Biologie Moléculaire et Cellulaire, 15 rue René Descartes, 67084 Strasbourg Cedex, France. Tel.: +33 388 41 70 51; Fax: +33 388 60 22 18; E-mail: p.romby@ibmc.u-strasbg.fr François Vandenesch, Faculté de Médecine Laennec, National Reference Center for Staphylococci, INSERM E0230, IFR62, 7 rue Guillaume Paradin, 69372 Lyon Cedex 08, France. Tel.: +33 478 77 86 57; Fax: +33 478 77 86 58; E-mail: denesch@univ-lyon1.fr 5 These authors contributed equally to this work Received 16 September 2004; Accepted 10 January 2005; Published online 27 January 2005. |
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| Abstract |
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| Staphylococcus aureus RNAIII is one of the largest regulatory RNAs, which controls several virulence genes encoding exoproteins and cell-wall-associated proteins. One of the RNAIII effects is the repression of spa gene (coding for the surface protein A) expression. Here, we show that spa repression occurs not only at the transcriptional level but also by RNAIII-mediated inhibition of translation and degradation of the stable spa mRNA by the double-strand-specific endoribonuclease III (RNase III). The 3' end domain of RNAIII, partially complementary to the 5' part of spa mRNA, efficiently anneals to spa mRNA through an initial loop–loop interaction. Although this annealing is sufficient to inhibit in vitro the formation of the translation initiation complex, the coordinated action of RNase III is essential in vivo to degrade the mRNA and irreversibly arrest translation. Our results further suggest that RNase III is recruited for targeting the paired RNAs. These findings add further complexity to the expression of the S. aureus virulon. |
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| Keywords: mRNA degradation, post-transcriptional control, regulatory RNA, RNase III, Staphylococcus aureus |
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