|
 |
|
|
| Subject Categories: Cell & Tissue Architecture | Signal Transduction |
|
| The EMBO Journal
(2005) 24, 742–752, doi:10.1038/sj.emboj.7600548 Published online 3 February 2005 |
|
ADAM10 cleavage of N-cadherin and regulation of cell–cell adhesion and  -catenin nuclear signalling |
|
|
| Karina Reiss1, 3, Thorsten Maretzky1, 3, Andreas Ludwig1, Thomas Tousseyn2, Bart de Strooper2, Dieter Hartmann2 and Paul Saftig1 |
|
|
1 Biochemical Institute, Christian-Albrechts-University Kiel, Kiel, Germany 2 Department for Human Genetics, KU Leuven and Flanders Interuniversity Institute for Biotechnology (VIB-4), Leuven, Belgium To whom correspondence should be addressed Paul Saftig, Biochemical Institute, Christian-Albrechts-University Kiel, Olshausenstr. 40, 24098 Kiel, Germany. Tel.: +49 431 880 2216; Fax: +49 431 880 2238; E-mail: psaftig@biochem.uni-kiel.de 3 These authors contributed equally to this work Received 23 August 2004; Accepted 10 December 2004; Published online 3 February 2005. |
|
|
|
| Abstract |
|
| Cadherins are critically involved in tissue development and tissue homeostasis. We demonstrate here that neuronal cadherin (N-cadherin) is cleaved specifically by the disintegrin and metalloproteinase ADAM10 in its ectodomain. ADAM10 is not only responsible for the constitutive, but also for the regulated, shedding of this adhesion molecule in fibroblasts and neuronal cells directly regulating the overall levels of N-cadherin expression at the cell surface. The ADAM10-induced N-cadherin cleavage resulted in changes in the adhesive behaviour of cells and also in a dramatic redistribution of -catenin from the cell surface to the cytoplasmic pool, thereby influencing the expression of -catenin target genes. Our data therefore demonstrate a crucial role of ADAM10 in the regulation of cell–cell adhesion and on -catenin signalling, leading to the conclusion that this protease constitutes a central switch in the signalling pathway from N-cadherin at the cell surface to -catenin/LEF-1-regulated gene expression in the nucleus. |
|
| Keywords: ADAM10, cell adhesion, N-cadherin, nuclear signalling, shedding |
|
|
|
Top of page MORE ARTICLES LIKE THISThese links to content published by NPG are automatically generated NEWS AND VIEWSNeurobiology Double trouble for neurons Nature News and Views (09 Oct 2003) Zipping together a cell adhesion interface Nature News and Views (23 Mar 1995) RESEARCHMetalloproteases regulate T-cell proliferation and effector function via LAG-3 The EMBO Journal Article (24 Jan 2007) The EMBO Journal Article (15 Apr 2002) |
|
|
| |
| |
| |
 | Email link to a friend |
| |
 | Download PDF |
| |
 | Full text |
| |
| |
| |
 | Next article |
| |
 | Previous article |
| |
 | Table of contents |
| |
| |
 | Rights and permissions |
| |
 | Reprints |
| |
| |
| |
 Register for table of contents by email | |
| |
| |
| |
