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| Subject Categories: RNA | Cellular Metabolism |
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| The EMBO Journal
(2005) 24, 580–588, doi:10.1038/sj.emboj.7600540 Published online 20 January 2005 |
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| Functional link between ribosome formation and biogenesis of iron–sulfur proteins |
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| Alexander Yarunin1, Vikram Govind Panse1, Elisabeth Petfalski2, Christophe Dez2, David Tollervey2 and Ed Hurt1 |
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1 Biochemie-Zentrum der Universität Heidelberg (BZH), Heidelberg, Germany 2 Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, UK To whom correspondence should be addressed Ed Hurt, Biochemie-Zentrum der Universität Heidelberg (BZH), Im Neuenheimer Feld 328, 69120 Heidelberg, Germany. Tel.: +49 6221 544173; Fax: +49 6221 544369; E-mail: cg5@ix.urz.uni-heidelberg.de Received 29 April 2004; Accepted 8 December 2004; Published online 20 January 2005. |
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| Abstract |
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| In genetic screens for ribosomal export mutants, we identified CFD1, NBP35 and NAR1 as factors involved in ribosome biogenesis. Notably, these components were recently reported to function in extramitochondrial iron–sulfur (Fe–S) cluster biosynthesis. In particular, Nar1 was implicated to generate the Fe–S clusters within Rli1, a potential substrate protein of unknown function. We tested whether the Fe–S protein Rli1 functions in ribosome formation. We report that rli1 mutants are impaired in pre-rRNA processing and defective in the export of both ribosomal subunits. In addition, Rli1p is associated with both pre-40S particles and mature 40S subunits, and with the eIF3 translation initiation factor complex. Our data reveal an unexpected link between ribosome biogenesis and the biosynthetic pathway of cytoplasmic Fe–S proteins. |
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| Keywords: eIF3, iron–sulfur cluster, Nar1, ribosome export, Rli1 |
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Top of page MORE ARTICLES LIKE THISThese links to content published by NPG are automatically generated NEWS AND VIEWSNature Structural & Molecular Biology News and Views (01 Nov 2005) Are DEAD-box proteins becoming respectable helicases? Nature Structural Biology News and Views (01 Feb 2000) RESEARCHThe EMBO Journal Article (05 Apr 2006) The ribosomal protein Rps15p is required for nuclear exit of the 40S subunit precursors in yeast The EMBO Journal Article (16 Jun 2004) |
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