Article
- The EMBO Journal (2005) 24, 4133 - 4143
- doi:10.1038/sj.emboj.7600873
Published online: 17 November 2005
Subject Category:
A role for BARS at the fission step of COPI vesicle formation from Golgi membrane
Jia-Shu Yang1,2, Stella Y Lee1,2, Stefania Spanò3, Helge Gad3, Leiliang Zhang1,2, Zhongzhen Nie4, Matteo Bonazzi3, Daniela Corda3, Alberto Luini3 and Victor W Hsu1,2
- Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, Boston, MA, USA
- Department of Medicine, Harvard Medical School, Boston, MA, USA
- Department of Cell Biology and Oncology, Consorzio Mario Negri Sud, Santa Maria Imbaro (Chieti), Italy
- Laboratory of Cellular Oncology, National Cancer Institute, Bethesda, MD, USA
Correspondence to:
Victor W Hsu, Brigham and Women's Hospital, One Jimmy Fund Way, Smith 538, Boston, MA 02115, USA. Tel.: +1 617 525 1103; Fax: +1 617 525 1104; E-mail: vhsu@rics.bwh.harvard.edu
Received 5 August 2005; Accepted 21 October 2005
Abstract
The core complex of Coat Protein I (COPI), known as coatomer, is sufficient to induce coated vesicular-like structures from liposomal membrane. In the context of biological Golgi membrane, both palmitoyl-coenzyme A (p-coA) and ARFGAP1, a GTPase-activating protein (GAP) for ADP-Ribosylation Factor 1, also participate in vesicle formation, but how their roles may be linked remains unknown. Moreover, whether COPI vesicle formation from Golgi membrane requires additional factors also remains unclear. We now show that Brefeldin-A ADP-Ribosylated Substrate (BARS) plays a critical role in the fission step of COPI vesicle formation from Golgi membrane. This role of BARS requires its interaction with ARFGAP1, which is in turn regulated oppositely by p-coA and nicotinamide adenine dinucleotide, which act as cofactors of BARS. Our findings not only identify a new factor needed for COPI vesicle formation from Golgi membrane but also reveal a surprising mechanism by which the roles of p-coA and GAP are linked in this process.
Keywords:
- ARFGAP1,
- BARS,
- COPI,
- membrane fission,
- vesicle formation
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