Introduction

The c-Myc (Myc) protein is an important regulator of many cellular processes, including growth, proliferation, differentiation and apoptosis (Pelengaris et al, 2002). These diverse cellular functions of Myc are closely tied to its ability to both activate and repress transcription (Pelengaris et al, 2002). Transcriptional activation by Myc occurs via dimerization with its partner Max and direct binding to specific DNA sequences, termed E-boxes. Myc stimulates gene expression in part at the level of chromatin, through its association with the cofactor TRRAP, thereby recruiting histone acetyltransferases such as GCN5 and Tip60 (Amati et al, 2001; Levens, 2003). Myc directly binds and stimulates the expression of a very large population of E-boxes containing genes (Levens, 2003).

In contrast to transcriptional activation by Myc, the mechanisms by which Myc silences gene expression are less well understood. An increasing number of target genes repressed by Myc have been identified, including the cyclin-dependent kinase inhibitors p21Cip1, p15Ink4b, p27Kip1 as well as genes involved in cellular differentiation and metabolism (Eisenman, 2001; Wanzel et al, 2003). Genes repressed by Myc do not seem to involve its direct association to DNA, but rather Myc is recruited to core promoters through protein–protein interactions with positively acting transcription factors, such as TFII-I (Roy et al, 1993), NF-Y (Izumi et al, 2001), and Miz-1 (Peukert et al, 1997). The current model for Myc-mediated gene silencing is that Myc associates with these activators and passively interferes with their transactivation function. The most convincingly demonstrated mechanism of functional interference by Myc is through its interaction with the Miz-1 transcription factor. Several studies have shown that Miz-1 binds and activates promoters of several genes, including p21Cip1 and p15Ink4b, and that transactivation by Miz-1 can be negatively regulated by its association with Myc (Seoane et al, 2001, 2002; Staller et al, 2001; Herold et al, 2002; van de Wetering et al, 2002). This is likely due, at least in part, because Myc competes with the coactivator p300 for binding to Miz-1 (Staller et al, 2001). In addition to functional interference with trancriptional activators, whether other mechanisms are involved in Myc-mediated gene silencing remains to be demonstrated.

DNA methylation at CpG dinucleotides is the major epigenetic modification in mammals and is known to be associated with transcriptional repression. This gene-silencing function can be related to the essential role played by CpG methylation for normal mammalian development (Jaenisch and Bird, 2003). The occurrence of DNA methylation within the genome is not random, but rather patterns of methylation are generated that are gene and tissue specific (Bird, 2002). How are DNA methylation patterns established is still poorly understood. Mechanistic insights into that question have begun to come from the characterization of the enzymes—the DNA methyltransferases—that generate methylation patterns. Three active DNA CpG methyltransferases, Dnmt1, Dnmt3a, and Dnmt3b, have been identified in mammals (Bestor et al, 1988; Okano et al, 1998). Whereas Dnmt3a and Dnmt3b have been shown to be required for de novo methylation (Okano et al, 1998, 1999), Dnmt1 appears to function primarily as a maintenance methyltransferase, restoring methylated cytosines following DNA replication (Leonhardt et al, 1992). Several studies have shown that Dnmts can act as corepressors to silence gene expression, in part through their association with histone deacetylases (Fuks et al, 2000, 2001; Robertson et al, 2000; Bachman et al, 2001), that help maintain chromatin in a compacted and silent state. DNA methyltransferases have little intrinsic sequence specificity beyond CpG dinucleotide (Yoder et al, 1997), and therefore other parameters are likely to be required to target their enzymatic activities to preferred genomic loci. It has been proposed that Dnmts may be directed by alterations in the chromatin structure, whereby chromosomal regions would not be equally accessible to Dnmts (Bird, 2002; Burgers et al, 2002). Consistent with this notion, studies of two SNF2 family helicases, ATRX and Lsh2, have shown that mutants of these enzymes decrease CpG methylation (Gibbons et al, 2000; Dennis et al, 2001). In addition, findings in Neurospora, Arabidopsis and more recently in mammals have shown that histone methylation at Lys9 of H3, which is associated with gene silencing, facilitates DNA methylation (Tamaru and Selker, 2001; Jackson et al, 2002; Lehnertz et al, 2003). Thus, chromatin modification or remodelling proteins could be needed for recruitment of Dnmts to particular loci. Another explanation to account for the varying DNA methylation patterns could involve a CpG methylation-targeting mechanism steered by sequence-specific binding proteins. Evidence for this mechanism has come from recent work showing an association of Dnmts with the oncogenic transcription factor PML-RAR, which binds to the RAR promoter and thereby recruits Dnmts to methylate and silence the targeted promoter (Di Croce et al, 2002).

In the present study, we report that Myc silences transcription by recruiting a DNA methyltransferase corepressor. We found that Myc associates with the Dnmt3a enzyme and targets its activity, through the DNA-binding protein Miz-1, to the p21Cip1 promoter. Recruitment of Dnmt3a by Myc leads to methylation and silencing of the targeted p21Cip1 gene. These data define a previously unrecognized pathway for Myc-mediated repression. In addition, our work sheds light on the poorly understood mechanisms by which specific CpG methylation patterns are established by DNA methyltransferases.

Results

Myc interacts with the corepressor Dnmt3a and associates with DNA methyltransferase activity

The mechanisms by which Myc silences gene expression remain unclear. Several studies indicate that Myc acts as a transcriptional repressor, at least in part, through its functional interference with transcriptional activators bound to different DNA sequences (Eisenman, 2001; Wanzel et al, 2003). In the present work, we considered whether Myc-mediated repression might in addition include an active mechanism involving the recruitment of corepressors. By means of an in vitro gluthatione S-transferase (GST) pull-down assay, we found that in vitro translated (IVT) and radiolabelled full-length Myc bound to the DNA methyltransferase Dnmt3a fused to GST (Figure 1A, left panel, lanes 4 and 5). Residues encompassing the conserved PHD-like motif of Dnmt3a were involved in the association with Myc (Figure 1A, left panel, lanes 4 and 5). In contrast, Myc failed to bind to the control GST alone or to the extreme N-terminal and C-terminal parts of Dnmt3a (Figure 1A, left panel, lanes 2, 3 and 6, respectively). We performed the reciprocal experiment using IVT full-length Dnmt3a and various GST fragments spanning the Myc protein. Figure 1A (right panel) shows that residues encompassing the conserved MBI and MBII domains of Myc contributed to its interaction with Dnmt3a.

To further validate the interaction between Myc and Dnmt3a, we used a coimmunoprecipitation approach. We cotransfected mammalian U2OS cells with vectors expressing full-length Myc tagged with HA and full-length Dnmt3a tagged with GAL4, and analyzed the cell lysates by immunoprecipitation using an antibody against GAL4 (for Dnmt3a), followed by Western blotting with an antibody against HA (for Myc). Figure 1B (left panel) indicates that Myc interacts with Dnmt3a (lane 1), whereas no precipitate was detected after transfection of either HA-Myc or GAL4-Dnmt3a alone (lanes 2 and 3, respectively). The reverse experiment, that is immunoprecipitation of HA-Myc followed by Western blotting for GAL4-Dnmt3a, also allowed specific association between the proteins (Figure 1B, right panel). The interaction between Myc and Dnmt3a can also be demonstrated in untransfected cells. In this experiment, an immunoprecipitate obtained with Dnmt3a-specific antibody was shown to contain Myc (Figure 1C, upper left panel, lane 1). As controls, no precipitation of Myc was observed using an unrelated CREB1 antibody (Figure 1C, upper left panel, lane 2). The presence of Dnmt3a or CREB1 in immunoprecipitates was visualized by Western blotting using anti-Dnmt3a or anti-CREB1, respectively (Figure 1C, bottom left panel). The reverse endogenous coimmunoprecipitation of Dnmt3a with Myc was also observed (Figure 1C, right panel).

The binding of Myc to Dnmt3a led us to expect that Myc would be associated with DNA methyltransferase activity. To test this, we evaluated whether antibodies against Myc could immunoprecipitate DNA methyltransferase activity from untransfected cells. As shown in Figure 1D, immunoprecipitation of endogenous Myc from HeLa nuclear extracts with anti-Myc antibodies purified significant amount of DNA methyltransferase activity (lane 2), whereas control immunoprecipitation with antibodies against another nuclear protein (PLZF; lane 1) showed background activity. The Dnmt enzymatic activity bound to Myc is provided by Dnmt3a. Indeed, when Myc fused to GST was incubated with bacterially expressed and active Dnmt3a followed by Dnmt enzymatic assay, Myc associated with Dnmt3a methyltransferase activity (Figure 1E). The Myc-associated enzymatic activity could also be due to Dnmt3b but not to Dnmt1, since, using a coimmunoprecipitation approach, we found that Myc coimmunoprecipitated with Dnmt3b but not with Dnmt1 after cotransfection into mammalian cells (see Supplementary Figure 1). Taken together, these data indicate that endogenous Myc binds to the Dnmt3a enzyme and is associated with DNA methyltransferase activity in vivo.

Dnmt3a specifically silences the Myc-repressed p21Cip1 gene

As Dnmt3a functions as a transcriptional corepressor (Bachman et al, 2001; Fuks et al, 2001), we next investigated whether Dnmt3a could act together with Myc to silence gene expression. We chose the p21Cip1 gene as a Myc-inhibited gene because it is a bona fide Myc-repressed target (Coller et al, 2000; Herold et al, 2002; Seoane et al, 2002) and it is known that its expression can be downregulated by DNA methylation (Allan et al, 2000; Zhu et al, 2003). Figure 2A (left panel) shows that transient transfection of p21Cip1 promoter together with increasing amounts of Myc led to a dose-dependent inhibition of its promoter activity. While expression of limiting amounts of Myc or Dnmt3a repressed p21Cip1 activity only slightly (Figure 2A, right panel, lanes 2 and 3), cotransfection of Myc along with Dnmt3a provided a synergistic repressive effect on p21Cip1 transcription (lane 5). High expression of Myc alone (Figure 2A, left panel, lane 4) leads to similar level of p21Cip1 repression observed by cotransfection of Myc and Dnmt3a (Figure 2A, right panel, lane 5). Hence, it was possible that enhancement of Myc-mediated p21Cip1 silencing by Dnmt3a (Figure 2A, right panel, lane 5) was simply due to increased expression of Myc protein levels after cotransfection of Dnmt3a. However, this is not the case, as shown by Western blotting of Myc protein after transfection of either high or low levels of Myc, in the presence or absence of Dnmt3a (Figure 2A, bottom panel). Together, these results suggest that Dnmt3a can act as a corepressor with Myc on the p21Cip1 promoter.

To establish whether endogenous Dnmt3a regulates the Myc-repressed p21Cip1 gene in vivo, we treated U2OS cells with a previously characterized Dnmt3a antisense oligonucleotide inhibitor (Robert et al, 2003). Quantitative real-time PCR analysis indicated that messenger RNA (mRNA) Dnmt3a levels were markedly decreased in cells treated with Dnmt3a antisense compared to mismatch control (Figure 2B, left panel). Similarly, Western blot analysis after treatment with Dnmt3a antisense showed specific depletion of Dnmt3a protein levels, whereas Dnmt3b or Dnmt1 protein levels were not affected (Figure 2B, left panel). As shown in Figure 2B (right panel), p21Cip1 mRNA levels were significantly elevated in the cells with reduced Dnmt3a levels. In contrast, the expression levels of ODC and NM23-H2, two E-box genes that are activated by Myc (Bello-Fernandez et al, 1993; Schuhmacher et al, 2001), were unchanged. The expression of S26, which is not regulated by Myc, was also not affected. Together, these data demonstrate that Dnmt3a is a specific repressor of the Myc-repressed p21Cip1 gene in vivo.

Myc targets Dnmt3a to the p21Cip1 promoter

We next asked whether Dnmt3a could be recruited by Myc to p21Cip1. To test this, we performed chromatin immunoprecipitation experiments (ChIPs), first on cells transfected with either the p21Cip1 promoter, and Dnmt3a alone, or in combination with an expression vector for HA-tagged Myc. We used primers located within the p21Cip1 proximal promoter region as it is the region recognized by Myc (Herold et al, 2002; Seoane et al, 2002; van de Wetering et al, 2002). Figure 3A shows that, in the presence of overexpressed Myc, Dnmt3a can bind to p21Cip1 (lane 2), whereas in the absence of exogenous Myc, Dnmt3a did not bind to the p21Cip1 proximal promoter (lane 6).

The above-mentioned ChIP data were obtained in transfected cells and may be considered as an artificial system. Thus, we next determine whether recruitment of Dnmt3a by Myc could also be observed from untransfected cells. To this end, we tested in ChIP assays the well-characterized c-myc knockout rat fibroblasts (c-myc-/-) and their wild-type counterparts (c-myc+/+) (Mateyak et al, 1997). In c-myc+/+ cells, the p21Cip1 proximal promoter is bound by Dnmt3a (Figure 3B, lane 2). However, in c-myc-/- cells, Dnmt3a binding is significantly reduced (Figure 3B, lane 6). Similar ChIPs on the albumin promoter, to which Myc does not bind (Zeller et al, 2001), show no Dnmt3a binding (Figure 3B, lower panel). Consistent with data presented in Figure 2B, the Myc-activated E-box promoters, ODC and NM23-H2, associated with Myc in c-myc+/+ cells but not with Dnmt3a (Figure 3C). Collectively, these data strongly suggest that Myc targets Dnmt3a selectively to the p21Cip1 promoter.

Myc and Dnmt3a corepress p21Cip1 promoter through association of Myc with Miz-1

Recent data indicated that Myc does not bind directly to the p21Cip1 proximal promoter but is recruited through its association with the DNA-binding protein Miz-1 (Herold et al, 2002; Seoane et al, 2002; van de Wetering et al, 2002). We therefore asked whether Miz-1 could be the factor that targets Myc and Dnmt3a to silence p21Cip1 expression. To test this idea, we first determined whether Dnmt3a can associate with Miz-1. As shown in Figure 4A, when Miz-1 and GAL4-tagged Dnmt3a expression vectors were transiently transfected in mammalian cells, we detected an interaction after immunoprecipitation with anti-GAL4 antibody, followed by Western blotting with an Miz-1-specific antibody. Dnmt3a also coimmunoprecipitated with Miz-1 in untransfected cells (Figure 4B). Further, immunoprecipitation of endogenous Miz-1 with anti-Miz-1 antibody purified significant DNA methyltransferase activity from HeLa nuclear extracts (Figure 4C, lane 2), whereas control immunoprecipitation using an irrelevant antibody (PLZF) gave background activity (Figure 4C, lane 1). These results indicate that Miz-1 can interact with the Dnmt3a DNA methyltransferase, consistent with its ability to associate with DNA methyltransferase activity in vivo. The Dnmt enzymatic activity bound to Miz-1 is provided by Dnmt3a (Figure 4F, see below) and also likely by Dnmt3b, but not Dnmt1. Indeed, coimmunoprecipitations after cotransfection into mammalian cells indicated that Miz-1 binds to Dnmt3b whereas Dnmt1 did not (see Supplementary Figure 2).

Several observations indicate that the interaction of Miz-1 with Dnmt3a is indirectly mediated by the binding of Dnmt3a to Myc. First, we performed a direct interaction assay by producing Dnmt3a, Myc, and Miz-1 as recombinant proteins in Escherichia coli. As shown in Figure 4D, GST-Myc bound histidine-tagged Dnmt3a (lane 2), whereas GST-Miz-1 (lane 4) did not or weakly. We used GST fused to the RP58 protein, a protein known to bind directly to Dnmt3a (Fuks et al, 2001), as a positive control (lane 3). Thus, Myc contacts Dnmt3a directly, while Miz-1 seems not. Second, similar coimmunoprecipitations as described in Figure 4A were performed, this time by transfecting limiting amounts of Miz-1 and GAL4-Dnmt3a, which resulted in only weak interaction between the two proteins (Figure 4E, lane 2). Overexpression of increasing amounts of Myc together with Miz-1 and GAL4-Dnmt3a strongly enhanced the association of Miz-1 with Dnmt3a (Figure 4E, lanes 3–6). As we cannot exclude that Miz-1 could weakly bind directly to Dnmt3a (Figure 4D, lane 4), it was possible that this enhanced Miz-1–Dnmt3a interaction was simply due to Myc expression increasing Dnmt3a levels. However, this possibility is unlikely since overexpression of Myc did not affect levels of Dnmt3a protein (Supplementary Figure 3). Following Dnmt3a-Miz-1 immunoprecipitation, we detected the presence of Myc by Western blotting using anti-Myc antibody (data not shown). Thus, these results suggest that Miz-1, Myc, and Dnmt3a can form a ternary complex. Next, and along the same line, DNA methyltransferase assays were performed using immobilized bacterially expressed GST-Miz-1 and recombinant Dnmt3a as a source of Dnmt enzyme, in the presence or absence of recombinant Myc. As shown in Figure 4F, addition of Myc significantly increased the association of Miz-1 with Dnmt3a enzymatic activity. This observation is consistent with coimmunoprecipitations presented in Figure 4E and suggests the formation of a trimeric Miz-1–Myc–Dnmt3a complex.

We then tested whether this ternary complex is involved in p21Cip1 transcriptional silencing. For this, we performed corepression assays similar to those described in Figure 2B, using a point mutant of Myc (Myc mut) that is deficient in binding to Miz-1 and thereby unable to repress p21Cip1 (Herold et al, 2002), while it still retains binding to Dnmt3a (Figure 4G, upper panel). Figure 4G (lower panel) shows that, while cotransfection of Dnmt3a together with Myc wt increased p21Cip1 inhibition in a cooperative manner (lane 5), coexpression of Dnmt3a along with the Myc point mutant strongly impaired the repression of p21Cip1 (lane 6). These results strongly suggest that corepression of p21Cip1 promoter activity by Dnmt3a and Myc is dependent on interaction of Myc with Miz-1.

DNA methylation participates in Myc-mediated silencing of p21Cip1

Having shown that Myc binds the Dnmt3a DNA methyltransferase and that the latter silences p21Cip1 expression, we next set out to establish whether DNA methylation was required for Myc to repress p21Cip1. To test this possibility, we first asked whether the repression mediated by Myc on p21Cip1 promoter linked to the luciferase gene could be relieved by the addition of the DNA methylation inhibitor 5-azacytidine (5-AZC). As shown in Figure 5A, the repressive effect observed with Myc on reporter activity was substantially relieved in cells treated with 5-AZC. As 5-AZC is a broad-spectrum DNA methyltransferase inhibitor, we used a point mutant of Dnmt3a that abolishes its enzymatic activity (Hsieh, 1999) and found that, as compared to wild-type Dnmt3a, the mutant is unable to repress the p21Cip1 promoter in cooperation with Myc (Figure 2A, right panel, lane 6).

The effect of 5-AZC on Myc-mediated silencing of p21Cip1 was verified in vivo by monitoring the expression of p21Cip1 in wild-type and knockout Myc rat fibroblasts that were treated or not with 5-AZC. RNA isolated from each cell type was reverse transcribed and amplified by polymerase chain reaction (RT–PCR). Figure 5B shows that, in the c-myc+/+ cells, p21Cip1 mRNA levels were elevated when cells were treated with 5-AZC, whereas the drug had no effect on the expression of an unrelated housekeeping gene, GAPDH. In contrast, in the c-myc-/- cells, the level of p21Cip1 mRNA was not affected by the addition of 5-AZC (Figure 5B). Reintroduction of Myc into knockout Myc cells (c-myc-/- +Myc) decreased p21Cip1 expression to a similar low level as observed in the parental c-myc+/+ cells, while 5-AZC treatment caused its re-expression (Figure 5B). These data strongly suggest that silencing of p21Cip1 by DNA methylation requires the presence of Myc. To confirm these observations and since 5-AZC can have pleiotropic effects (Christman et al, 1985), we next carried out bisulphite genomic sequencing to compare the methylation status of p21Cip1 proximal promoter between c-myc+/+ and c-myc-/- cells. Figure 5C indicates that several CpGs located within the p21Cip1 proximal promoter showed differential methylation between c-myc+/+ and c-myc-/- cells (CpGs situated outside this cluster region did not show significant difference in methylation status; data not shown). Further, c-myc-/- cells in which Myc expression had been restored (c-myc-/- +Myc) gained methylation of p21Cip1, thus confirming that the presence of Myc is needed, at least in part, for DNA methylation of p21Cip1 proximal promoter. These observations do not seem to be restricted to Rat1 fibroblasts as U2OS cells, which express Myc (data not shown), also show methylation of p21Cip1 promoter (Figure 5D).

Discussion

Myc silences transcription by active recruitment of DNA methyltransferase corepressor

Recent studies indicate that transcriptional silencing by Myc significantly contributes to most of its biological functions; yet, the details underlying this process are still unclear. To date, Myc-mediated gene repression is known to involve a passive mechanism through interference with transcriptional activators (Eisenman, 2001; Wanzel et al, 2003). Here, we report a new mechanism by which Myc silences gene expression through recruitment of the Dnmt3a corepressor to a promoter, thereby leading to subsequent DNA methylation and silencing of the targeted promoter.

On Myc-repressed genes, Myc does not seem to bind directly to DNA but rather is recruited through protein–protein interaction with other transcription factors. As mentioned above, in the case of the p21Cip1 gene, recent studies demonstrated that Myc binds to p21Cip1 promoter through its association with the DNA-binding factor Miz-1 (Herold et al, 2002; Seoane et al, 2002; van de Wetering et al, 2002). The binding of Myc with Miz-1 switches Miz-1 from a transcriptional activator to a repressor of p21Cip1 (Herold et al, 2002; Seoane et al, 2002; van de Wetering et al, 2002), likely by preventing the interaction of Miz-1 with its own coactivator (Seoane et al, 2001; Staller et al, 2001). Our studies suggest that silencing of p21Cip1 by Myc and Dnmt3a involves the association of Myc with Miz-1. A model could be envisaged in which Myc switches Miz-1 from a transcriptional activator to a repressor by a dual mechanism: (i) as reported by others (Herold et al, 2002; Seoane et al, 2002), Myc prevents recruitment of a coactivator to Miz-1, and (ii) Myc brings the corepressor Dnmt3a to Miz-1. These two mechanisms could be particularly important to keep tight control over p21Cip1 regulation. The ability of Myc/Miz-1 to deliver methyltransferase activity may be relevant for any gene silenced by the Myc/Miz-1 complex. Thus, repression of the p15ink4b gene, a known Myc/Miz-1 target (Seoane et al, 2001; Staller et al, 2001) which is regulated by DNA methylation (Herman et al, 1996), may also involve the recruitment of DNA methyltransferase corepressor.

Mechanisms by which DNA methylation is targeted to preferred genomic sequences

How DNA methyltransferases establish DNA methylation patterns within a cell remains unclear. DNA methyltransferases have little sequence specificity beyond CpG dinucleotide (Yoder et al, 1997) and several mechanisms could be envisaged to explain the regional specificity they exhibit. One possibility could be that chromatin modifications or remodelling proteins are required for targeting Dnmts to particular loci. Studies in Neurospora, Arabidopsis and more recently in mammals revealed that histone methylation at Lys9 of H3 can direct methylation of DNA (Tamaru and Selker, 2001; Jackson et al, 2002; Lehnertz et al, 2003). In Arabidopsis, the adaptor protein LHP1, which binds with high affinity to histone H3 when methylated at Lys9, was found to interact with the CMT3 DNA methyltransferase (Jackson et al, 2002). Similarly, in mammals, Dnmts were found to physically associate with HP1 (Fuks et al, 2003a; Lehnertz et al, 2003). In addition, HP1 was recently reported to be essential for DNA methylation in Neurospora (Freitag et al, 2004). Hence, it was proposed that histone methylation would influence DNA methylation through the adaptor HP1, which would recruit DNA methyltransferases to CpG that has to be methylated (Jackson et al, 2002; Fuks et al, 2003a; Lehnertz et al, 2003).

Besides the proposed recruitment of Dnmts by chromatin-based mechanisms, the present work provides evidence that DNA methylation patterns can be regulated by the targeting of Dnmts to particular loci through their association with specific transcription factors. Our finding supports and extends our observations that the PML-RAR transcription factor associates with Dnmts, thereby allowing CpG methylation of its target gene (Di Croce et al, 2002). The targeting of DNA methyltransferases to specific loci by transcriptional regulators is reminiscent of mechanisms by which chromatin-modifying enzymes establish local changes in chromatin structure to regulate gene expression. Indeed, recruitment to promoters of histone acetyltransferases and deacetylases as well as histone methyltransferases by transcription factors seems to be a common and general strategy to bring their enzymatic activities to targeted genes (Kouzarides, 2002; Kurdistani and Grunstein, 2003). It is therefore likely that targeting of DNA methyltransferases to precise genes through their interaction with specific transcription factors may be a wide and general mechanism by which DNA methylation is generated at preferred loci.

Materials and methods

Plasmid constructions

We cloned the following sequences by PCR using appropriate sets of primers: full-length Dnmt3a wild-type or catalytic mutant (Hsieh, 1999), full-length Dnmt1 or Dnmt3b into pcDNA3.1-GAL4, full-length Dnmt3a wild-type or full-length Myc into pET-30 (Novagen) and Dnmt3a fragments into the vector pGEX (Pharmacia). The following plasmids have been described previously: pGEX-Dnmt3a 286–661, pGEX-Dnmt3a 490–582 and pGEX-RP58 (Fuks et al, 2001), pGEX-Miz-1 269-803, pGEX-Myc deletion constructs (Hateboer et al, 1993), pcDNA3-Myc wt and pcDNA3-Myc mut (Myc V394D point mutant deficient in Miz-1 binding) (Herold et al, 2002), pcDNA3 Miz-1 (Peukert et al, 1997), pcDNA3-HA-Myc (Frank et al, 2001), pBJ Myc (Frank et al, 2001) and p21Cip1-Luc reporter (2.4 kb).

GST fusion and histine-tagged proteins, in vitro translation, pull-down and direct interaction assays

Recombinant proteins were expressed in and purified from E. coli Top10 or Bl21 as described (Fuks et al, 2000). In vitro translation reactions and GST pull-down experiments have been described previously (Fuks et al, 2000). Dnmt3a, Myc wt or Myc mut were IVT from pcDNA3-GAL4-Dnmt3a, pcDNA3-Myc wt or pcDNA3-Myc mut, respectively. Direct interaction assays were performed as described (Fuks et al, 2001), using anti-His antibody (H1029, Sigma).

Cell culture, transfections and luciferase assays

Cell lines were maintained in DMEM supplemented with 8% fetal calf serum and grown at 37°C, 5% CO₂. The c-myc+/+ (TGR1) and c-myc-/- (HO15.19) Rat1 fibroblasts (Mateyak et al, 1997) were a generous gift of J Sedivy. The 'c-myc-/- +Myc' cells are c-myc-/- cells infected, as described (Frank et al, 2001), with a pBabe retrovirus expressing Myc. Transfections were performed using polyethylene imine (PEI) (Euromedex) as described previously (Deplus et al, 2002). Luciferase assays were performed with the Promega luciferase Assay System. Transfection efficiencies were normalized using a cotransfected plasmid encoding for -galactosidase, which is measured in a -galactosidase assay kit (Tropix). When used, 5-azacytidine was added 4 h following transfection (2 M final; Sigma). The results shown are the average of at least three independent experiments with error bars displaying standard deviations.

Antisense treatment and quantitative real-time PCR

U2OS were treated for 3 h with 50 nM of the already described 2'-O-methylphosphorothioate Dnmt3a antisense or mismatch control oligonucleotides (Robert et al, 2003), in OptiMEM (Gibco) supplemented with 8.5 g/ml LIPOFECTAMINE Reagent (Invitrogen). Cells were then incubated for 24 h in complete medium. RNA was extracted by Tripure reagent, reverse-transcribed and analysed by real-time PCR as described (Loriot et al, 2003). Specific primers and probes for the amplification are available on request. The expression levels of the genes of interest were normalized to the expression level of -actin. The results shown are the average of three independent experiments with error bars displaying standard deviations. Statistical analyses were carried out using the Wilcoxon–Mann–Whitney test and significance was assigned at P<0.05. For control Western blotting, we used antibodies against Dnmt1 (a gift from S Pradhan), Dnmt3a or Dnmt3b (a gift from E Li) and actin (A5316, Sigma).

Immunoprecipitations and Western blot analysis

293 or U2OS cells were transiently transfected in culture dishes (10 cm diameter) with a total of 6 g of plasmids as described (Deplus et al, 2002). Standard procedures were used for coimmunoprecipitations and Western blotting (Deplus et al, 2002). For endogenous immunoprecipitations, antibodies were incubated with HeLa nuclear extracts (4C Biotech) in IPH buffer (Fuks et al, 2000) at 4°C overnight. Antibodies used were against HA (12C5A, Roche), GAL4 (5C1, Santa Cruz), Myc (C33, Santa Cruz), Miz-1 (N17, Santa Cruz), CREB1 (24H4B, Santa Cruz) and Dnmt3a (IMG268; Imgenex).

DNA methyltransferase assay

DNA methyltransferase assays were carried out as described (Fuks et al, 2000). For immunoprecipitations preceding the DNA methyltransferase assay, we used antibodies from Santa Cruz against: Myc (C33), PLZF (F15) and Miz-1 (N17). For assays using recombinant and active Dnmt3a, we used bacterially expressed His-tagged Dnmt3a and immobilized GST-Myc 1–204 or GST-Miz-1 269–803. Where appropriated, eluted His-tagged Myc was also used.

RNA purification and RT–PCR analysis

Rat1 fibroblasts (Mateyak et al, 1997) were treated or not with 5-azacytidine as described before (Allan et al, 2000). Extraction of total RNA was carried out using Tripure reagent (Roche) according to the manufacturer's instructions. In all, 2 g of RNA was reversed transcribed using random hexamers (Amersham/Pharmacia Biotech) and SuperscriptII reverse transcriptase (Life Technologies Inc.). PCR was achieved with Taq DNA polymerase (Promega) for 30–35 cycles of amplification.

Chromatin immunoprecipitation (ChIP)

ChIP was performed from either 293 transfected cells (one 6 cm diameter dish) or untransfected rat1 fibroblasts (one 15 cm diameter dish). Cells were crosslinked with formaldehyde (0.75%; Sigma) at room temperature for 10 min. Cells were rinsed twice with ice-cold PBS (pH 7.4) and collected in PBS. ChIPs were then performed essentially as described (Fuks et al, 2003b). We used anti-HA antibodies (12C5A, Roche), anti-GAL4 (5C1, Santa Cruz) or an unrelated antibody (anti-GFP, ab290, Abcam) in 293 cells, and anti-Myc (C33 or N262, Santa Cruz), anti-Dnmt3a (IMG268, Imgenex) or anti-HA (12C5A, Roche) in Rat1 cells. Immunoprecipitated DNA was analysed by PCR for the presence of human p21Cip1 proximal promoter (-427 bp versus start site to +16). For rat p21Cip1 proximal promoter, primers from -250 bp versus start site to +142 were used. Primers for albumin promoter (Zeller et al, 2001), and the E-box promoters ODC (Frank et al, 2001) and NM23-H2 (Frank et al, 2001), were described previously. The cycle number and the amount of template were varied to ensure that results were within the PCR linear range.

Bisulphite genomic sequencing

Methylation status of the p21Cip1 promoter in Rat1 fibroblasts or U2OS was assessed by bisulphite genomic sequencing. BamHI- or EcoRI-digested genomic DNA (5 g) from these cells was subjected to sodium bisulphite modification and the p21Cip1 promoter was amplified as described (Di Croce et al, 2002). The amplified product was subcloned into the pCR2.1 vector by TA cloning (Invitrogen) and sequenced via automated sequencing. Primer sequences for rat p21Cip1 amplification were as follows: sense strand 4149 5'-TGGTTTTTATTTGGGTAGTAGTTG-3', antisense strand 4766 5'-CCTACCTCCAATTCCCCTTAACTC-3'; sense strand 4163 5'-GTAGTAGTTGTTAAAAGGATTTTG-3', antisense strand 4741 5'-AACACTATAACAACTCACACCTCT-3'. Primer sequences for human p21Cip1 amplification were as follows: sense strand 5'-AAAAGTTAGATTTGTGGTTTATTT-3', antisense strand 5'-TCTCACCTCCTCTAAATACCTC-3'; sense strand 5'-GGGAGGAGGGAAGTGTTTTT -3', antisense strand 5'-CAACTACTCACACCTCAACTAAC-3'.

Supplementary data

Supplementary data are available at The EMBO Journal Online.

Acknowledgements

We thank J Sedivy for the rat1 c-myc+/+ (TGR-1) and c-myc-/- (HO15.19) cells, M Eilers for Miz-1 reagents, W El Deiry for the p21Cip1-Luc reporter plasmid, S Pradhan for anti-Dnmt1 antibody and E Li for Dnmt3a and Dnmt3b immune sera. We thank P Putmans for excellent technical assistance. CB and EV were supported by the Belgian Télévie; RD and CD were funded by the FRIA and FNRS, respectively, and DB was funded by the 'Research in Brussels' action. FF is a 'Chercheur Qualifié du FNRS' from the Belgian Fonds National de la Recherche Scientifique. This work was funded by grants from 'Fundacio La Caixa' to LDC and from the 'Fédération Belge contre le Cancer', the FNRS, a grant from 'FB Assurances' and from the 'Action de Recherche Concertée de la Communauté Française de Belgique' to YdL and FF.

References

Allan LA, Duhig T, Read M, Fried M (2000) The p21(WAF1/CIP1) promoter is methylated in Rat-1 cells: stable restoration of p53-dependent p21(WAF1/CIP1) expression after transfection of a genomic clone containing the p21(WAF1/CIP1) gene. Mol Cell Biol 20: 1291−1298 | Article |

Amati B, Frank SR, Donjerkovic D, Taubert S (2001) Function of the c-Myc oncoprotein in chromatin remodeling and transcription. Biochim Biophys Acta 1471: M135−M145

Bachman KE, Rountree MR, Baylin SB (2001) Dnmt3a and Dnmt3b are transcriptional repressors that exhibit unique localization properties to heterochromatin. J Biol Chem 276: 32282−32287 | Article |

Bello-Fernandez C, Packham G, Cleveland JL (1993) The ornithine decarboxylase gene is a transcriptional target of c-Myc. Proc Natl Acad Sci USA 90: 7804−7808

Bestor T, Laudano A, Mattaliano R, Ingram V (1988) Cloning and sequencing of a cDNA encoding DNA methyltransferase of mouse cells. The carboxyl-terminal domain of the mammalian enzymes is related to bacterial restriction methyltransferases. J Mol Biol 203: 971−983

Bird A (2002) DNA methylation patterns and epigenetic memory. Genes Dev 16: 6−21 | Article |

Burgers WA, Fuks F, Kouzarides T (2002) DNA methyltransferases get connected to chromatin. Trends Genet 18: 275−277 | Article |

Christman JK, Schneiderman N, Acs G (1985) Formation of highly stable complexes between 5-azacytosine-substituted DNA and specific non-histone nuclear proteins. Implications for 5-azacytidine-mediated effects on DNA methylation and gene expression. J Biol Chem 260: 4059−4068

Coller HA, Grandori C, Tamayo P, Colbert T, Lander ES, Eisenman RN, Golub TR (2000) Expression analysis with oligonucleotide microarrays reveals that MYC regulates genes involved in growth, cell cycle, signaling, and adhesion. Proc Natl Acad Sci USA 97: 3260−3265 | Article |

Dennis K, Fan T, Geiman T, Yan Q, Muegge K (2001) Lsh, a member of the SNF2 family, is required for genome-wide methylation. Genes Dev 15: 2940−2944 | Article |

Deplus R, Brenner C, Burgers WA, Putmans P, Kouzarides T, de Launoit Y, Fuks F (2002) Dnmt3L is a transcriptional repressor that recruits histone deacetylase. Nucleic Acids Res 30: 3831−3838 | Article |

Di Croce L, Raker VA, Corsaro M, Fazi F, Fanelli M, Faretta M, Fuks F, Lo Coco F, Kouzarides T, Nervi C, Minucci S, Pelicci PG (2002) Methyltransferase recruitment and DNA hypermethylation of target promoters by an oncogenic transcription factor. Science 295: 1079−1082 | Article |

Eisenman RN (2001) Deconstructing myc. Genes Dev 15: 2023−2030 | Article |

Frank SR, Schroeder M, Fernandez P, Taubert S, Amati B (2001) Binding of c-Myc to chromatin mediates mitogen-induced acetylation of histone H4 and gene activation. Genes Dev 15: 2069−2082 | Article |

Freitag M, Hickey PC, Khlafallah TK, Read ND, Selker EU (2004) HP1 is essential for DNA methylation in neurospora. Mol Cell 13: 427−434 | Article |

Fuks F, Burgers WA, Brehm A, Hughes-Davies L, Kouzarides T (2000) DNA methyltransferase Dnmt1 associates with histone deacetylase activity. Nat Genet 24: 88−91 | Article |

Fuks F, Burgers WA, Godin N, Kasai M, Kouzarides T (2001) Dnmt3a binds deacetylases and is recruited by a sequence-specific repressor to silence transcription. EMBO J 20: 2536−2544 | Article |

Fuks F, Hurd PJ, Deplus R, Kouzarides T (2003a) The DNA methyltransferases associate with HP1 and the SUV39H1 histone methyltransferase. Nucleic Acids Res 31: 2305−2312 | Article |

Fuks F, Hurd PJ, Wolf D, Nan X, Bird AP, Kouzarides T (2003b) The methyl-CpG-binding protein MeCP2 links DNA methylation to histone methylation. J Biol Chem 278: 4035−4040 | Article |

Gibbons RJ, McDowell TL, Raman S, O'Rourke DM, Garrick D, Ayyub H, Higgs DR (2000) Mutations in ATRX, encoding a SWI/SNF-like protein, cause diverse changes in the pattern of DNA methylation. Nat Genet 24: 368−371 | Article |

Hateboer G, Timmers HT, Rustgi AK, Billaud M, van't Veer LJ, Bernards R (1993) TATA-binding protein and the retinoblastoma gene product bind to overlapping epitopes on c-Myc and adenovirus E1A protein. Proc Natl Acad Sci USA 90: 8489−8493

Herman JG, Jen J, Merlo A, Baylin SB (1996) Hypermethylation-associated inactivation indicates a tumor suppressor role for p15INK4B. Cancer Res 56: 722−727

Herold S, Wanzel M, Beuger V, Frohme C, Beul D, Hillukkala T, Syvaoja J, Saluz HP, Haenel F, Eilers M (2002) Negative regulation of the mammalian UV response by Myc through association with Miz-1. Mol Cell 10: 509−521 | Article |

Hsieh CL (1999) In vivo activity of murine de novo methyltransferases, Dnmt3a and Dnmt3b. Mol Cell Biol 19: 8211−8218

Izumi H, Molander C, Penn LZ, Ishisaki A, Kohno K, Funa K (2001) Mechanism for the transcriptional repression by c-Myc on PDGF beta-receptor. J Cell Sci 114: 1533−1544

Jackson JP, Lindroth AM, Cao X, Jacobsen SE (2002) Control of CpNpG DNA methylation by the KRYPTONITE histone H3 methyltransferase. Nature 416: 556−560 | Article |

Jaenisch R, Bird A (2003) Epigenetic regulation of gene expression: how the genome integrates intrinsic and environmental signals. Nat Genet 33 (Suppl): 245−254 | Article |

Kouzarides T (2002) Histone methylation in transcriptional control. Curr Opin Genet Dev 12: 198−209 | Article |

Kurdistani SK, Grunstein M (2003) Histone acetylation and deacetylation in yeast. Nat Rev Mol Cell Biol 4: 276−284 | Article |

Lehnertz B, Ueda Y, Derijck AA, Braunschweig U, Perez-Burgos L, Kubicek S, Chen T, Li E, Jenuwein T, Peters AH (2003) Suv39h-mediated histone H3 lysine 9 methylation directs DNA methylation to major satellite repeats at pericentric heterochromatin. Curr Biol 13: 1192−1200 | Article |

Leonhardt H, Page AW, Weier HU, Bestor TH (1992) A targeting sequence directs DNA methyltransferase to sites of DNA replication in mammalian nuclei. Cell 71: 865−873 | Article |

Levens DL (2003) Reconstructing MYC. Genes Dev 17: 1071−1077 | Article |

Loriot A, Boon T, De Smet C (2003) Five new human cancer-germline genes identified among 12 genes expressed in spermatogonia. Int J Cancer 105: 371−376 | Article |

Mateyak MK, Obaya AJ, Adachi S, Sedivy JM (1997) Phenotypes of c-Myc-deficient rat fibroblasts isolated by targeted homologous recombination. Cell Growth Differ 8: 1039−1048

Okano M, Bell DW, Haber DA, Li E (1999) DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development. Cell 99: 247−257 | Article |

Okano M, Xie S, Li E (1998) Cloning and characterization of a family of novel mammalian DNA (cytosine-5) methyltransferases. Nat Genet 19: 219−220 | Article |

Pelengaris S, Khan M, Evan G (2002) c-MYC: more than just a matter of life and death. Nat Rev Cancer 2: 764−776 | Article |

Peukert K, Staller P, Schneider A, Carmichael G, Hanel F, Eilers M (1997) An alternative pathway for gene regulation by Myc. EMBO J 16: 5672−5686 | Article |

Robert MF, Morin S, Beaulieu N, Gauthier F, Chute IC, Barsalou A, MacLeod AR (2003) DNMT1 is required to maintain CpG methylation and aberrant gene silencing in human cancer cells. Nat Genet 33: 61−65 | Article |

Robertson KD, Ait-Si-Ali S, Yokochi T, Wade PA, Jones PL, Wolffe AP (2000) DNMT1 forms a complex with Rb, E2F1 and HDAC1 and represses transcription from E2F-responsive promoters. Nat Genet 25: 338−342 | Article |

Roy AL, Carruthers C, Gutjahr T, Roeder RG (1993) Direct role for Myc in transcription initiation mediated by interactions with TFII-I. Nature 365: 359−361 | Article |

Schuhmacher M, Kohlhuber F, Holzel M, Kaiser C, Burtscher H, Jarsch M, Bornkamm GW, Laux G, Polack A, Weidle UH, Eick D (2001) The transcriptional program of a human B cell line in response to Myc. Nucleic Acids Res 29: 397−406 | Article |

Seoane J, Le HV, Massague J (2002) Myc suppression of the p21(Cip1) Cdk inhibitor influences the outcome of the p53 response to DNA damage. Nature 419: 729−734 | Article |

Seoane J, Pouponnot C, Staller P, Schader M, Eilers M, Massague J (2001) TGFbeta influences Myc, Miz-1 and Smad to control the CDK inhibitor p15INK4b. Nat Cell Biol 3: 400−408 | Article |

Staller P, Peukert K, Kiermaier A, Seoane J, Lukas J, Karsunky H, Moroy T, Bartek J, Massague J, Hanel F, Eilers M (2001) Repression of p15INK4b expression by Myc through association with Miz-1. Nat Cell Biol 3: 392−399 | Article |

Tamaru H, Selker EU (2001) A histone H3 methyltransferase controls DNA methylation in Neurospora crassa. Nature 414: 277−283 | Article |

van de Wetering M, Sancho E, Verweij C, de Lau W, Oving I, Hurlstone A, van der Horn K, Batlle E, Coudreuse D, Haramis AP, Tjon-Pon-Fong M, Moerer P, van den Born M, Soete G, Pals S, Eilers M, Medema R, Clevers H (2002) The beta-catenin/TCF-4 complex imposes a crypt progenitor phenotype on colorectal cancer cells. Cell 111: 241−250 | Article |

Wanzel M, Herold S, Eilers M (2003) Transcriptional repression by Myc. Trends Cell Biol 13: 146−150 | Article |

Yoder JA, Soman NS, Verdine GL, Bestor TH (1997) DNA (cytosine-5)-methyltransferases in mouse cells and tissues. Studies with a mechanism-based probe. J Mol Biol 270: 385−395 | Article |

Zeller KI, Haggerty TJ, Barrett JF, Guo Q, Wonsey DR, Dang CV (2001) Characterization of nucleophosmin (B23) as a Myc target by scanning chromatin immunoprecipitation. J Biol Chem 276: 48285−48291

Zhu WG, Srinivasan K, Dai Z, Duan W, Druhan LJ, Ding H, Yee L, Villalona-Calero MA, Plass C, Otterson GA (2003) Methylation of adjacent CpG sites affects Sp1/Sp3 binding and activity in the p21(Cip1) promoter. Mol Cell Biol 23: 4056−4065 | Article |