Article
- The EMBO Journal (2005) 24, 2379 - 2390
- doi:10.1038/sj.emboj.7600711
Published online: 9 June 2005
Subject Category:
Altered nucleosome occupancy and histone H3K4 methylation in response to 'transcriptional stress'
Lian Zhang1, Stephanie Schroeder1,a, Nova Fong1 and David L Bentley1
- Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, UCHSC at Fitzsimons, Aurora, CO, USA
Correspondence to:
David L Bentley, Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, UCHSC at Fitzsimons, Mail Stop 8101, PO Box 6511, Aurora, CO 80045, USA. Tel.: +1 303 724 3238; Fax: +1 303 724 3215; E-mail: david.bentley@uchsc.edu
aPresent address: Department of Biological Sciences, Webster University, St Louis, MO 63119, USA
Received 6 December 2004; Accepted 18 May 2005
Abstract
We report that under 'transcriptional stress' in budding yeast, when most pol II activity is acutely inhibited, rapid deposition of nucleosomes occurs within genes, particularly at 3' positions. Whereas histone H3K4 trimethylation normally marks 5' ends of highly transcribed genes, under 'transcriptional stress' induced by 6-azauracil (6-AU) and inactivation of pol II, TFIIE or CTD kinases Kin28 and Ctk1, this mark shifted to the 3' end of the TEF1 gene. H3K4Me3 at 3' positions was dynamic and could be rapidly removed when transcription recovered. Set1 and Chd1 are required for H3K4 trimethylation at 3' positions when transcription is inhibited by 6-AU. Furthermore, 
chd1 suppressed the growth defect of set1. We suggest that a 'transcriptional stress' signal sensed through Set1, Chd1, and possibly other factors, causes H3K4 hypermethylation of newly deposited nucleosomes at downstream positions within a gene. This response identifies a new role for H3K4 trimethylation at the 3' end of the gene, as a chromatin mark associated with impaired pol II transcription.
Keywords:
- Chd1,
- chromatin,
- histone H3K4 methylation,
- Set1,
- transcription
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