Article
- The EMBO Journal (2005) 24, 1874 - 1885
- doi:10.1038/sj.emboj.7600659
Published online: 5 May 2005
Subject Categories:
Developmental specificity of auxin response by pairs of ARF and Aux/IAA transcriptional regulators
Dolf Weijers1,a, Eva Benkova1,a, Katja E Jäger1,b, Alexandra Schlereth1, Thorsten Hamann1,c, Marika Kientz1, Jill C Wilmoth2, Jason W Reed2 and Gerd Jürgens1
- ZMBP Entwicklungsgenetik, Universität Tübingen, Tübingen, Germany
- Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
Correspondence to:
Gerd Jürgens, ZMBP Entwicklungsgenetik, Universität Tübingen, Auf der Morgenstelle 3, 72076 Tübingen, Germany. Tel.: +49 7071 297 8887; Fax: +49 7071 297 5797; E-mail: gerd.juergens@zmbp.uni-tuebingen.de
aThese authors contributed equally to this work
bPresent address: Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK
cPresent address: Department of Plant Biology, Carnegie Institution of Washington, Stanford, CA 94305, USA
Received 18 January 2005; Accepted 1 April 2005
Abstract
The plant hormone auxin elicits many specific context-dependent developmental responses. Auxin promotes degradation of Aux/IAA proteins that prevent transcription factors of the auxin response factor (ARF) family from regulating auxin-responsive target genes. Aux/IAAs and ARFs are represented by large gene families in Arabidopsis. Here we show that stabilization of BDL/IAA12 or its sister protein IAA13 prevents MP/ARF5-dependent embryonic root formation whereas stabilized SHY2/IAA3 interferes with seedling growth. Although both bdl and shy2-2 proteins inhibited MP/ARF5-dependent reporter gene activation, shy2-2 was much less efficient than bdl to interfere with embryonic root initiation when expressed from the BDL promoter. Similarly, MP was much more efficient than ARF16 in this process. When expressed from the SHY2 promoter, both shy2-2 and bdl inhibited cell elongation and auxin-induced gene expression in the seedling hypocotyl. By contrast, gravitropism and auxin-induced gene expression in the root, which were promoted by functionally redundant NPH4/ARF7 and ARF19 proteins, were inhibited by shy2-2, but not by bdl protein. Our results suggest that auxin signals are converted into specific responses by matching pairs of coexpressed ARF and Aux/IAA proteins.
Keywords:
- Arabidopsis,
- ARF transcription factor,
- Aux/IAA protein,
- embryo,
- root gravitropism
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